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Mouse IL-5(Interleukin 5) ELISA Kit

Citations(18)Uniprot : P04401

Cat.No.:E-EL-M0722
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse IL-5 in samples. No significant cross-reactivity or interference between Mouse IL-5 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IL-5 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-5. You can calculate the concentration of Mouse IL-5 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-5 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-5 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	50.34	86.56	464.22	51.71	92.29	432.78
Standard deviation	2.80	5.01	24.37	3.55	4.08	15.41
CV (%)	5.56	5.79	5.25	6.87	4.42	3.56

Recovery

The recovery of Mouse IL-5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-102	96
EDTA plasma (n=8)	91-104	97
Cell culture media (n=8)	87-102	94

Linearity

Samples were spiked with high concentrations of Mouse IL-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P04401
Synonyms	IL5, EDF, TRF, E-CSF, ESGF
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
Aerofloral studies and allergenicity of dominant pollen types in Taraba and Bauchi States of Northeastern Nigeria

DOI: 10.1016/j.scitotenv.2022.157076

PMID: 35780899
Sample: plasma
FOOD CHEMISTRY (2021) IF: 7.514
A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols

DOI: 10.1016/j.foodchem.2021.131589

Sample: Spleen cells
Food & Function (2022) IF: 6.317
Peanut allergen induces more serious allergic reactions than other allergens involving MAPK signaling pathways

DOI: 10.1039/D2FO00777K

PMID: 35920097
Sample: serum
Cell Death Discovery (2022) IF: 5.241
IL-37 isoform D acts as an inhibitor of soluble ST2 to boost type 2 immune homeostasis in white adipose tissue

DOI: 10.1038/s41420-022-00960-3

PMID: 35383145
Sample: Serum
Nutrients (2021) IF: 5.719
Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

DOI: 10.3390/nu13093209

PMID: 34579088
Sample: Splenocyte
Clinical and Translational Allergy (2022) IF: 5.871
MiR-29b regulates Th2 cell differentiation in asthma by targeting inducible B7-H3 and STAT3

DOI: 10.1002/clt2.12114

PMID: 35079347
Sample: bronchoalveolar lavage fluid (BALF),Lung tissue
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice

DOI: 10.3390/ijms23010226

PMID: 35008651
Sample: Splenocyte
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020) IF: 4.192
Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

DOI: 10.1021/acs.jafc.0c00461

PMID: 32182051
Sample: Serum,Cell culture supernatant
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
In vitro and in vivo relaxation and anti-inflammation of natural flavonoids from Elaeagnus pungens leaf via L-type calcium channel and targeting MAPK signal pathway

DOI: 10.1016/j.jep.2021.114071

PMID: 33831464
Sample: Cell culture supernatant
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes‐associated protein

DOI: 10.1111/jcmm.15485

Sample: Tissue homogenate
Nutrients (2020) IF: 4.546
Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

DOI: 10.3390/nu12010249

PMID: 31963703
Sample: Serum,Cell culture supernatant
INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway

DOI: 10.1016/j.intimp.2021.108266

PMID: 34678694
Sample: BALF
INFLAMMATION (2020) IF: 3.212
Casticin Attenuates Osteoarthritis-Related Cartilage Degeneration by Inhibiting the ROS-Mediated NF-κB Signaling Pathway in vitro and in vivo

DOI: 10.1007/s10753-019-01167-y

Sample: serum,ADTC5 cell
PHARMACOLOGY (2023) IF: 3.429
DPP4 Inhibitor Sitagliptin Reduces Inflammatory Responses and Mast Cell Activation in Allergic Rhinitis

DOI: 10.1159/000528634

Sample: serum，nasal lavage fluid
INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
Borneol inhibits CD4 + T cells proliferation by down-regulating miR-26a and miR-142-3p to attenuate asthma

DOI: 10.1016/j.intimp.2020.107223

Sample: Bronchoalveolar Lavage Fluid
Dose-Response (2021) IF: 2.658
Plasma Proteins As Biodosimetric Markers of Low-Dose Radiation in Mice:

DOI: 10.1177/15593258211016257

Sample: Plasma
Molecular Medicine Reports (2019) IF: 2.952
BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice

DOI: 10.3892/mmr.2019.10780

Sample: BMDCs
AEROBIOLOGIA (2018) IF: 1.515
Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria

DOI: 10.1007/s10453-018-9533-7

Sample: Plasma

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