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Mouse PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit

Citations(9)Uniprot : P37238

Cat.No.:E-EL-M0893
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse PPAR-γ in samples. No significant cross-reactivity or interference between Mouse PPAR-γ and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse PPAR-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PPAR-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PPAR-γ. You can calculate the concentration of Mouse PPAR-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse PPAR-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse PPAR-γ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.55	1.57	4.57	0.56	1.43	4.45
Standard deviation	0.03	0.08	0.14	0.04	0.06	0.20
CV (%)	5.45	5.10	3.06	7.14	4.20	4.49

Recovery

The recovery of Mouse PPAR-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-98	91
EDTA plasma (n=8)	89-105	96
Cell culture media (n=8)	88-101	95

Linearity

Samples were spiked with high concentrations of Mouse PPAR-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P37238
Research Area	Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Neuroscience, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
4-Thiazolidinone-based derivatives do not affect differentiation of mouse embryo fibroblasts (3T3-L1 cell line) into adipocytes

DOI: 10.1016/j.cbi.2021.109538

PMID: 34097888
Sample: 3T3-L1 cells
Food & Function (2022) IF: 5.396
Black corn (Zea mays L.) whole flour improved the antioxidant capacity and prevented the adipogenesis in mice fed a high-fat diet.

DOI: 10.1039/D1FO04205J

Sample: liver
NEUROCHEMISTRY INTERNATIONAL (2022) IF: 4.297
Calcium channel antagonists interfere with the mechanism of action of elastin-derived peptide VGVAPG in mouse cortical astrocytes in vitro

DOI: 10.1016/j.neuint.2022.105405

PMID: 35934159
Sample: astrocyte cell
NEUROTOXICITY RESEARCH (2019) IF: 3.311
The Elastin-Derived Peptide VGVAPG Does Not Activate the Inflammatory Process in Mouse Cortical Astrocytes In Vitro

DOI: 10.1007/s12640-019-00114-x

Sample: mouse astrocyte cell
TOXICOLOGY IN VITRO (2021) IF: 3.5
Triclosan affects the expression of nitric oxide synthases (NOSs), peroxisome proliferator-activated receptor gamma (PPARγ), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in mouse neocortical neurons in vitro

DOI: 10.1016/j.tiv.2021.105143

PMID: 33722737
Sample: Cell culture supernatant
Journal of Personalized Medicine (2022) IF: 3.508
Artichoke (Cynara Scolymus) Methanolic Leaf Extract Alleviates Diethylnitrosamine-Induced Toxicity in BALB/c Mouse Brain: Involvement of Oxidative Stress and Apoptotically Related Klotho/PPARγ Signaling

DOI: 10.3390/jpm12122012

Sample: brain
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2019) IF: 2.11
The effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice

DOI: 10.1080/13813455.2019.1648517

Sample: adipose
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2018) IF: 2.238
Impact of elastin-derived VGVAPG peptide on bidirectional interaction between peroxisome proliferator-activated receptor gamma (Pparγ) and beta-galactosidase (β-Gal) expression in mouse cortical astrocytes in vitro

DOI: 10.1007/s00210-018-1591-4

Sample: Tissue homogenate
PharmaNutrition (2022)
Digested protein from chia seed (Salvia hispanica L) prevents obesity and associated inflammation of adipose tissue in mice fed a high-fat diet

DOI: 10.1016/j.phanu.2022.100298

Sample: adipose tissue

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