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Mouse S100B(S100 Calcium Binding Protein B) ELISA Kit

Citations(7)Uniprot : P50114

Cat.No.:E-EL-M1033
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	78.13-5000 pg/mL
Sensitivity	46.88 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse S100B in samples. No significant cross-reactivity or interference between Mouse S100B and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse S100B concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse S100B. You can calculate the concentration of Mouse S100B in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse S100B were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse S100B were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	269.60	555.80	1940.80	279.50	608.10	1946.90
Standard deviation	13.50	32.80	100.90	19.30	31.60	91.50
CV (%)	5.01	5.90	5.20	6.91	5.20	4.70

Recovery

The recovery of Mouse S100B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-105	96
EDTA plasma (n=8)	91-106	99
Cell culture media (n=8)	92-105	99

Linearity

Samples were spiked with high concentrations of Mouse S100B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P50114
Synonyms	S100-B, NEF, S100Beta
Research Area	Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ACS Nano (2018) IF: 15.881
Inducing a Transient Increase in Blood–Brain Barrier Permeability for Improved Liposomal Drug Therapy of Glioblastoma Multiforme

DOI: 10.1021/acsnano.8b03785

Sample: plasma
MOLECULAR THERAPY (2021) IF: 11.454
Prior nasal delivery of antagomiR-122 prevents radiation-induced brain injury

DOI: 10.1016/j.ymthe.2021.06.019

PMID: 34174438
Sample: hippocampus
Pharmaceuticals (2020) IF: 4.286
Kinin B2 Receptor Activation Prevents the Evolution of Alzheimer’s Disease Pathological Characteristics in a Transgenic Mouse Model

DOI: 10.3390/ph13100288

Sample: Cell culture supernatant
INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
The receptor for advanced glycation end products mediates dysfunction of airway epithelial barrier in a lipopolysaccharides-induced murine acute lung injury model

DOI: 10.1016/j.intimp.2021.107419

PMID: 33548580
Sample: Bronchoalveolar Lavage Fluid
PLoS One (2021) IF: 3.24
Key role of Extracellular RNA in hypoxic stress induced myocardial injury

DOI: 10.1371/journal.pone.0260835

PMID: 34882718
Sample: plasma
JOURNAL OF NEUROIMMUNOLOGY (2021) IF: 3.478
Hippocampal microglia CD40 mediates NPSLE cognitive dysfunction in mice

DOI: 10.1016/j.jneuroim.2021.577620

PMID: 34062352
Sample: Serum,Urine
Frontiers in Aging Neuroscience (2019) IF: 3.633
Neuroprotective Effects of Kinin B2 Receptor in Organotypic Hippocampal Cultures of Middle-Aged Mice

DOI: 10.3389/fnagi.2019.00168

Sample: hippocampal

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