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Mouse VCAM-1/CD106(Vascular cell adhesion molecule 1) ELISA Kit

Citations(7)Uniprot : P29533

Cat.No.:E-EL-M1233
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Mouse VCAM-1/CD106 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VCAM-1/CD106. You can calculate the concentration of Mouse VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse VCAM-1/CD106 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse VCAM-1/CD106 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.10	4.30	0.50	1.00	4.40
Standard deviation	0.03	0.06	0.23	0.03	0.04	0.16
CV (%)	6.00	5.45	5.35	6.00	4.00	3.64

Recovery

The recovery of Mouse VCAM-1/CD106 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-98	93
EDTA plasma (n=8)	85-95	90
Cell culture media (n=8)	88-103	96

Target Information

Database Links	SwissProt: P29533
Research Area	Cancer, Cardiovascular, Neuroscience, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Theranostics (2019) IF: 8.0630
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

DOI: 10.7150/thno.31461

Sample: plasma
Frontiers in Cardiovascular Medicine (2021) IF: 6.05
Potential Mechanism of Dingji Fumai Decoction Against Atrial Fibrillation Based on Network Pharmacology, Molecular Docking, and Experimental Verification Integration Strategy

DOI: 10.3389/fcvm.2021.712398

PMID: 34859062
Sample: HL-1 cells
Cancers (2020) IF: 6.126
Retinol-Binding Protein 4 Accelerates Metastatic Spread and Increases Impairment of Blood Flow in Mouse Mammary Gland Tumors

DOI: 10.3390/cancers12030623

Sample: plasma
BIOORGANIC CHEMISTRY (2022) IF: 5.275
Twelve novel sesquiterpenes with anti-inflammatory and cholesterol-lowering activities from burdock leaves

DOI: 10.1016/j.bioorg.2022.105940

PMID: 35749853
Sample: RAW264.7 cell
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2017) IF: 5.476
A novel mechanism of diabetic vascular endothelial dysfunction: Hypoadiponectinemia-induced NLRP3 inflammasome activation

DOI: 10.1016/j.bbadis.2017.02.012

Sample: Serum,Cell culture supernatant
Frontiers in Pharmacology (2022) IF: 5.811
Blocking VCAM-1 Prevents Angiotensin II-Induced Hypertension and Vascular Remodeling in Mice.

DOI: 10.3389/fphar.2022.825459

Sample: serum
MOLECULAR PHARMACEUTICS (2018) IF: 4.556
Combination Therapy with DETA/NO and Clopidogrel Inhibits Metastasis in Murine Mammary Gland Cancer Models via Improved Vasoprotection

DOI: 10.1021/acs.molpharmaceut.8b00781

PMID: 30234992
Sample: Plasma,Tissue homogenate

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