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Mouse VWF(Von Willebrand Factor) ELISA Kit

Citations(6)Uniprot : Q8CIZ8

Cat.No.:E-EL-M1247
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.25-80 ng/mL
Sensitivity	0.75 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse VWF in samples. No significant cross-reactivity or interference between Mouse VWF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse VWF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VWF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VWF. You can calculate the concentration of Mouse VWF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse VWF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse VWF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.33	12.59	29.76	4.41	13.12	27.83
Standard deviation	0.23	0.75	1.48	0.30	0.64	1.45
CV (%)	5.31	5.96	4.97	6.80	4.88	5.21

Recovery

The recovery of Mouse VWF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-101	92
EDTA plasma (n=8)	93-104	99
Cell culture media (n=8)	91-102	97

Linearity

Samples were spiked with high concentrations of Mouse VWF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q8CIZ8
Synonyms	F8VWF, VWD
Research Area	Cancer, Cardiovascular, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Theranostics (2019) IF: 8.0630
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

DOI: 10.7150/thno.31461

Sample: plasma
Neuroscience Bulletin (2021) IF: 5.203
Acrolein Induces Systemic Coagulopathy via Autophagy-dependent Secretion of von Willebrand Factor in Mice after Traumatic Brain Injury

DOI: 10.1007/s12264-021-00681-0

PMID: 33939120
Sample: Plasma
MOLECULAR PHARMACEUTICS (2018) IF: 4.556
Combination Therapy with DETA/NO and Clopidogrel Inhibits Metastasis in Murine Mammary Gland Cancer Models via Improved Vasoprotection

DOI: 10.1021/acs.molpharmaceut.8b00781

PMID: 30234992
Sample: Plasma,Tissue homogenate
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY (2019) IF: 3.323
ADAMTS13 protects mice against renal ischemia-reperfusion injury by reducing inflammation and improving endothelial function

DOI: 10.1152/ajprenal.00405.2018

PMID: 30461292
Sample: Plasma,Tissue homogenate
Frontiers in Pediatrics (2022) IF: 3.569
Platelets are indispensable for alveolar development in neonatal mice

DOI: 10.3389/fped.2022.943054

PMID: 36016884
Sample: blood
JOURNAL OF CELLULAR BIOCHEMISTRY (2018) IF: 2.959
mTOR/STAT‐3 pathway mediates mesenchymal stem cell–secreted hepatocyte growth factor protective effects against lipopolysaccharide‐induced vascular endothelial barrier dysfunction and apoptosis

DOI: 10.1002/jcb.27642

Sample: Cell culture supernatant

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Reviews/Q&A

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Reviews
Q&A

e****dSubmitted [ Jan 25 2023 ]

Asked: Is this specific for the mouse VWF protein or is it for VWF A2 or VWF antigen 2? Is it possible to buy just the capture / detecting antibodies?

adminSubmitted [ Jan 25 2023 ]

Answered: This is specific for the mouse VWF protein, and the capture / detecting antibodies is not sold separately.

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