NCI-H2228 [H2228; H-2228] Cell Line

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      Catalog number:EP-CL-0570

      Size:
      • 1×10⁶ cells/vial
      Qty:
      - +
      Price: $449

      Lead Time: 3-5 daysWelcome to order from local distributors.

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      Origin and General Characteristics

      Cell Name NCI-H2228 [H2228; H-2228] Cell Line
      Age Female
      Tissue Lung
      Morphology Epithelial
      Growth Properties Adherent
      Descriptions The line was established in August 1989 from lung adenocarcinoma. The tissue donor was a non-smoker.
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium RPMI-1640 +10% FBS +Glutamax+1% P/S
      Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
      Subcultivation Ratio 1:2-1:4
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
      Storage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%
      Temperature: 37℃

      Duration

      It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

      Related Resources

      Recommendations for handling of cryopreserved cells

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