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AFP Polyclonal Antibody

Cat:E-AB-40293
Manual MSDS

Price: $ 380

Price: $ 230

Price: $ 125

Price: $ 65

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Hep G2,Mouse placenta,Rat placenta,Mouse Fetal liver,Rat Fetal liver,Mouse liver,Mouse kidney,Rat liver,Rat kidney
Verified Samples in IHC:Mouse liver,Human liver cancer,Human ovarian cancer, MCF-7, MCF-7,Human liver cancer
Dilution

WB:1:500-1:1000, IHC 1:200-1:500, IF 1:100-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant Mouse Afp protein expressed by E.coli
Abbre AFP
Synonyms Afp; AFPD; Alpha fetoglobulin; Alpha fetoprotein precursor; Alpha-1-fetoprotein; FETA; Hereditary persistence of alpha fetoprotein; HPAFP
Swissprot
Calculated MW 67 kDa
Observed MW 73 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted
Concentration 1.1mg/mL
Buffer PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
Purification Method Antigen Affinity Purification
Research Areas Cancer; Cardiovascular; Developmental Biology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.