TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as Ready-to-Use mono-component liquids that require no preparation before use. The product mainly contains 3,3’,5,5’-tetramethylbenzidine and contains no DMF or DMSO.
One-component TMB Substrate yields a blue color (Amax= 370 nm and 652 nm) when reacted with peroxidase in microwell applications. The product changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Method for use

1. Add 100 μL of the TMB Substrate Solution to each microplate well.
2. Incubate for 5~30 min according to the reaction system.
3. Stop reaction by adding 50 uL stop solution (1M HCl or 1M H2SO4).
4. Measure the absorbance of each well at 450 nm.

Storage

2-8℃, 12 months
Shading light.

Citations

Nanomaterials (2022) IF: 5.719
Surface-Enhanced Carboxyphenyl Diazonium Functionalized Screen-Printed Carbon Electrode for the Screening of Tuberculosis in Sputum Samples

DOI: 10.3390/nano12152551

PMID: 35893519
Vaccines (2021) IF: 4.422
K-Ras Peptide Mimotope Induces Antigen Specific Th1 and B-Cell Immune Responses against G12A-Mutated K-Ras Antigen in Balb/c Mice

DOI: 10.3390/vaccines9030195

PMID: 33652552
Sample: intra and extra-cellular total protein samples fro
Tropical Medicine and Infectious Disease (2022) IF: 3.711
Non-Invasive Detection of SARS-CoV-2 Antigen in Saliva versus Nasopharyngeal Swabs Using Nanobodies Conjugated Gold Nanoparticles

DOI: 10.3390/tropicalmed7060102

PMID: 35736981
PeerJ (2018) IF: 2.118
In silico-guided sequence modifications of K-ras epitopes improve immunological outcome against G12V and G13D mutant KRAS antigens

DOI: 10.7717/peerj.5056

Sample: Serum
BIOSCIENCE REPORTS (2019) IF: 2.535
Tumor suppressor properties of the small C-terminal domain phosphatases in non-small cell lung cancer

DOI: 10.1042/BSR20193094

PMID: 31774910
Sample: Cell lysate

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