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Peroxidase (POD) Activity Assay Kit (Plant Samples)

Cat:E-BC-K227-S Citations (6)
Manual MSDS

Price: $ 220

Price: $ 150

Size:
100Assays 50Assays
Quantity:
  • Sample Type: plant tissue
  • Detection Instrument: Spectrophotometer(240 nm、420 nm)
  • Reasearch Areas: Oxidative stress
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Product Details
Detection Principle The peroxidase can catalyze the decomposition of H2O2 and produce water and oxygen. And oxygen oxidized pyrogallic acid to form yellow product. The activity of peroxidase can be calculated by measuring the absorbance at 420 nm.
Synonyms POD
Sample Type plant tissue
Sensitivity 0.5 U/mL
Detection Range 0.5-40 U/mL
Detection Method Colorimetric method
Assay Time 60 min
Precision inter-assay CV: 5.5% | intra-assay CV: 3.8%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
10% Chinese rose tissue homogenization 1
10% Epipremnum aureum tissue homogenization 2-3
10% Green pepper tissue homogenization 1
10% Mushrooms tissue homogenization 1
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.