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PGE2(Prostaglandin E2) ELISA Kit

Citations(129)

  • Cat.No.:E-EL-0034

  • Reactivity: Universal
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Manual MSDS

To Purchase E-EL-0034

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes PGE2 in samples. No significant cross-reactivity or interference between PGE2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of PGE2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE2. During the reaction, PGE2 in the sample or standard competes with a fixed amount of PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PGE2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PGE2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 101.30 236.80 779.30 96.00 259.90 728.60
Standard deviation 6.60 11.60 42.10 4.90 13.80 31.30
CV (%) 6.52 4.90 5.40 5.10 5.31 4.30

Recovery

The recovery of PGE2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-109 99
EDTA plasma(n=8) 87-99 94
Cell culture media(n=8) 86-96 91

Linearity

Samples were spiked with high concentrations of PGE2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsPG-E2, Dinoprostone
Research AreaSignal Transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
elisa assay procedure 2

2. Aspirate and wash the plate for 3 times
elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 5

5. Add 50μL Stop Solution
elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Cell Metabolism (2022) IF: 31.373
    Cancer cell-intrinsic XBP1 drives immunosuppressive reprogramming of intratumoral myeloid cells by promoting cholesterol production

    DOI: 10.1016/j.cmet.2022.10.010

    PMID: 36351432

    		 Sample: MDSCs,B16 cell,MC38 cell
  2. Journal for ImmunoTherapy of Cancer (2020) IF: 10.252
    Melanoma-specific bcl-2 promotes a protumoral M2-like phenotype by tumor-associated macrophages

    DOI: 10.1136/jitc-2019-000489

    PMID: 32269145

    		 Sample: Cell sample
  3. Science Immunology (2019) IF: 10.551
    ATP release drives heightened immune responses associated with hypertension

    DOI: 10.1126/sciimmunol.aau6426
    Sample: plasma
  4. CARBOHYDRATE POLYMERS (2022) IF: 10.723
    Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism

    DOI: 10.1016/j.carbpol.2022.120032

    PMID: 36184177

    		 Sample: bronchoalveolar fluid,RAW264.7 cell
  5. Journal of Crohns & Colitis (2015) IF: 9.071
    Diet-Induced Hyperhomocysteinaemia Increases Intestinal Inflammation in an Animal Model of Colitis

    DOI: 10.1093/ecco-jcc/jjv094
    Sample: Plasma
  6. eLife (2020) IF: 7.08
    Increase of circulating IGFBP-4 following genotoxic stress and its implication for senescence

    DOI: 10.7554/eLife.54523
    Sample: MSC
  7. FREE RADICAL BIOLOGY AND MEDICINE (2021) IF: 7.376
    Multiple 'omics'-analysis reveals the role of prostaglandin E2 in Hirschsprung's disease

    DOI: 10.1016/j.freeradbiomed.2020.12.456

    PMID: 33465467

    		 Sample: Tissue homogenate(colon and uterus)
  8. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Xiaojianzhong decoction attenuates gastric mucosal injury by activating the p62/Keap1/Nrf2 signaling pathway to inhibit ferroptosis

    DOI: 10.1016/j.biopha.2022.113631

    PMID: 36122518

    		 Sample: gastric mucosal tissue,
  9. Frontiers in Immunology (2021) IF: 7.561
    Treatment With Endothelin-A Receptor Antagonist BQ123 Attenuates Acute Inflammation in Mice Through T-Cell-Dependent Polymorphonuclear Myeloid-Derived Suppressor Cell Activation

    DOI: 10.3389/fimmu.2021.641874

    PMID: 33828553

    		 Sample: Cell lysate
  10. Journal of Materials Chemistry B (2022) IF: 7.571
    Polydopamine-modified decellularized intestinal scaffolds loaded with adipose-derived stem cells promote intestinal regeneration

    DOI: 10.1039/D2TB01389D
  11. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021) IF: 6.291
    Stereological analysis and transcriptome profiling of testicular injury induced by di-(2-ethylhexyl) phthalate in prepubertal rats

    DOI: 10.1016/j.ecoenv.2021.112326

    PMID: 34015638

    		 Sample: Serum
  12. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 6.291
    In utero exposure to DBP stimulates release of GnRH by increasing the secretion of PGE2 in the astrocytes of the hypothalamus in the offspring mice

    DOI: 10.1016/j.ecoenv.2020.110698

    PMID: 32388187

    		 Sample: Cell culture supernatant
  13. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2021) IF: 6.514
    N-2-(phenylamino) benzamide derivatives as novel anti-glioblastoma agents: Synthesis and biological evaluation

    DOI: 10.1016/j.ejmech.2021.113817

    PMID: 34537445

    		 Sample: brain tissue
  14. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Pharmacological evaluation of continentalic acid for antidiabetic potential

    DOI: 10.1016/j.biopha.2021.111411

    PMID: 33711550

    		 Sample: Whole Blood
  15. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53
    Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role

    DOI: 10.1016/j.biopha.2020.110075

    PMID: 32179202

    		 Sample: Tissue homogenate
  16. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53
    A study of nanometre aggregates formation mechanism and antipyretic effect in Bai-Hu-Tang, an ancient Chinese herbal decoction

    DOI: 10.1016/j.biopha.2020.109826

    PMID: 31978766

    		 Sample: Tissue homogenate
  17. Cells (2019) IF: 6.6
    Anti-Neuroinflammatory Effect of Alantolactone through the Suppression of the NF-κB and MAPK Signaling Pathways

    DOI: 10.3390/cells8070739
    Sample: PC12 cells,BV2 cells
  18. PHYTOMEDICINE (2022) IF: 6.656
    A discovery of clinically approved Panlongqi Tablet for repositioning to treat osteoarthritis by inhibiting PI3K/AKT activation

    DOI: 10.1016/j.phymed.2022.154360
    Sample: serum
  19. Journal of Inflammation Research (2021) IF: 6.922
    Anti-Inflammatory Effects of Four Psilocybin-Containing Magic Mushroom Water Extracts in vitro on 15-Lipoxygenase Activity and on Lipopolysaccharide-Induced Cyclooxygenase-2 and Inflammatory Cytokines in Human U937 Macrophage Cells

    DOI: 10.2147/JIR.S317182
    Sample: U937 cell
  20. Frontiers in Immunology (2020) IF: 5.085
    High Expression of IL-36γ in Influenza Patients Regulates Interferon Signaling Pathway and Causes Programmed Cell Death During Influenza Virus Infection

    DOI: 10.3389/fimmu.2020.552606

    PMID: 33193319

    		 Sample: Plasma
  21. INFLAMMOPHARMACOLOGY (2022) IF: 5.093
    Cyclooxygenase-2 activates EGFR–ERK1/2 pathway via PGE2-mediated ADAM-17 signaling in testosterone-induced benign prostatic hyperplasia

    DOI: 10.1007/s10787-022-01123-7
    Sample: prostatic tissue
  22. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo

    DOI: 10.1016/j.jep.2022.115846

    PMID: 36280015

    		 Sample: RAW264.7 cells
  23. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Exploring the anti-inflammatory potential of Colocasia esculenta root extract in in-vitro and in-vivo models of inflammation

    DOI: 10.1016/j.jep.2022.116021
    Sample: serum,RAW 264.7 cells
  24. Pharmaceuticals (2022) IF: 5.215
    Aspalathus linearis (Rooibos) and Agmatine May Act Synergistically to Beneficially Modulate Intestinal Tight Junction Integrity and Inflammatory Profile

    DOI: 10.3390/ph15091097

    PMID: 36145318

    		 Sample: HT-29 cell
  25. PHYTOMEDICINE (2021) IF: 5.34
    Bioactive constituents of Mosla chinensis-cv. Jiangxiangru ameliorate inflammation through MAPK signaling pathways and modify intestinal microbiota in DSS-induced colitis mice

    DOI: 10.1016/j.phymed.2021.153804

    PMID: 34735907

    		 Sample: colon tissue
  26. Foods (2022) IF: 5.561
    Freshwater Clam Extract Attenuates Indomethacin-Induced Gastric Damage In Vitro and In Vivo

    DOI: 10.3390/foods12010156
    Sample: gastric mucosa
  27. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS (2021) IF: 5.571
    Dual action gels containing DsiRNA loaded gold nanoparticles: Augmenting diabetic wound healing by promoting angiogenesis and inhibiting infection

    DOI: 10.1016/j.ejpb.2021.09.007

    PMID: 34582971

    		 Sample: serum
  28. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2020) IF: 5.573
    Design, synthesis and bioactivity study of N-salicyloyl tryptamine derivatives as multifunctional agents for the treatment of neuroinflammation

    DOI: 10.1016/j.ejmech.2020.112217
    Sample: BV2 cell,C6 cell
  29. SCIENCE OF THE TOTAL ENVIRONMENT (2019) IF: 5.589
    Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus

    DOI: 10.1016/j.scitotenv.2019.02.419

    PMID: 30833257

    		 Sample: Plasma
  30. Frontiers in Pharmacology (2022) IF: 5.811
    Effects of Pereskia aculeate Miller Petroleum Ether Extract on Complete Freund’s Adjuvant-Induced Rheumatoid Arthritis in Rats and its Potential Molecular Mechanisms

    DOI: 10.3389/fphar.2022.869810
    Sample: serum
  31. Frontiers in Pharmacology (2022) IF: 5.988
    Pharmacological investigation of brucine anti-ulcer potential

    DOI: 10.3389/fphar.2022.886433

    PMID: 36059979

    		 Sample: stomach tissue
  32. Frontiers in Pharmacology (2022) IF: 5.988
    Pharmacological basis of bergapten in gastrointestinal diseases focusing on H+/K+ ATPase and voltage-gated calcium channel inhibition: A toxicological evaluation on vital organs

    DOI: 10.3389/fphar.2022.1005154
    Sample: stomach tissue
  33. Frontiers in Pharmacology (2022) IF: 5.988
    Effect of Rumex dentatus on Gastrointestinal Protection and Toxicology in Rodents via Investigating H+/K+-ATPase, Calcium Channels, and PDE Mediated Signaling.

    DOI: 10.3389/fphar.2022.936161
    Sample: Stomach tissue
  34. Frontiers in Pharmacology (2022) IF: 5.988
    Aconiti lateralis radix praeparata total alkaloids exert anti-RA effects by regulating NF-κB and JAK/STAT signaling pathways and promoting apoptosis.

    DOI: 10.3389/fphar.2022.980229
    Sample: MH7A cell
  35. JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020) IF: 4.018
    Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling

    DOI: 10.1016/j.jri.2020.103192

    PMID: 32950783

    		 Sample: Cell culture supernatant
  36. INFLAMMATION (2021) IF: 4.092
    Benzoylaconine Modulates LPS-Induced Responses Through Inhibition of Toll-Like Receptor-Mediated NF-κB and MAPK Signaling in RAW264.7 Cells

    DOI: 10.1007/s10753-021-01478-z
    Sample: RAW264.7 cells
  37. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020) IF: 4.192
    Chrysin Inhibits Melanoma Tumor Metastasis via Interfering with the FOXM1/β-Catenin Signaling

    DOI: 10.1021/acs.jafc.0c03123

    PMID: 32797754

    		 Sample: Cell culture medium
  38. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Protective effect of the combination of essential oil from patchouli and tangerine peel against gastric ulcer in rats

    DOI: 10.1016/j.jep.2021.114645

    PMID: 34530094

    		 Sample: serum
  39. CANCER SCIENCE (2018) IF: 4.372
    Pretreatment with probiotic Bifico ameliorates colitis‐associated cancer in mice: Transcriptome and gut flora profiling

    DOI: 10.1111/cas.13497
    Sample: serum
  40. Scientific Reports (2021) IF: 4.38
    Vitamin D stimulates miR-26b-5p to inhibit placental COX-2 expression in preeclampsia

    DOI: 10.1038/s41598-021-90605-9

    PMID: 34045549

    		 Sample: Tissue homogenate
  41. MOLECULES (2022) IF: 4.412
    The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced Fever

    DOI: 10.3390/molecules27082465

    PMID: 35458665

    		 Sample: serum
  42. MOLECULES (2022) IF: 4.412
    Metabolomic Profiling Reveals Protective Effects and Mechanisms of Sea Buckthorn Sterol against Carbon Tetrachloride-Induced Acute Liver Injury in Rats

    DOI: 10.3390/molecules27072224

    PMID: 35408620

    		 Sample: Liver tissues
  43. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Phillygenin inhibited LPS-induced RAW 264.7 cell inflammation by NF-κB pathway

    DOI: 10.1016/j.ejphar.2021.174043
    Sample: Cell culture supernatant
  44. Journal of Functional Foods (2021) IF: 4.451
    Q43, a new triterpenoid extracted from Chinese acorn, exhibits pronounced anti-neuroinflammatory activity through the MAPK and NF-κB pathways

    DOI: 10.1016/j.jff.2021.104566
    Sample: BV2 cells
  45. TOXICOLOGY AND APPLIED PHARMACOLOGY (2023) IF: 4.46
    Tyramine-induced gastrointestinal dysregulation is attenuated via estradiol associated mechanisms in a zebrafish larval model

    DOI: 10.1016/j.taap.2023.116399
    Sample: larvae
  46. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Exploring the role of Aquaporins (AQPs) in LPS induced systemic inflammation and the ameliorative effect of Garcinia in male Wistar rat

    DOI: 10.1007/s10787-021-00832-9

    PMID: 34106384

    		 Sample: serum
  47. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Comparison of individual and combination treatments with naproxen, prednisolone and hydroxychloroquine to treat Complete Freund’s Adjuvant induced arthritis

    DOI: 10.1007/s10787-021-00875-y
    Sample: serum
  48. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Chemical characterization and anti-arthritic appraisal of Monotheca buxifolia methanolic extract in Complete Freund’s Adjuvant-induced arthritis in Wistar rats

    DOI: 10.1007/s10787-020-00783-7
    Sample: serum
  49. INFLAMMOPHARMACOLOGY (2022) IF: 4.473
    Alterations to microbial secretome by estrogen may contribute to sex bias in irritable bowel syndrome

    DOI: 10.1007/s10787-021-00906-8

    PMID: 35022916

    		 Sample: Colon adenocarcinoma (HT-29) cell
  50. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Huoxuezhitong capsule ameliorates MIA-induced osteoarthritis of rats through suppressing PI3K/ Akt/ NF-κB pathway

    DOI: 10.1016/j.biopha.2020.110471
    Sample: Serum
  51. AMERICAN JOURNAL OF CHINESE MEDICINE (2021) IF: 4.667
    Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

    DOI: 10.1142/S0192415X21500452

    PMID: 33827384

    		 Sample: peyer’s patches (PPs),RAW 264.7 cell
  52. Biomedicines (2022) IF: 4.757
    The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model

    DOI: 10.3390/biomedicines10102508

    PMID: 36289770

    		 Sample: liver tissue
  53. Aging-US (2020) IF: 4.831
    NO up-regulates migraine-related CGRP via activation of an Akt/GSK-3β/NF-κB signaling cascade in trigeminal ganglion neurons

    DOI: 10.18632/aging.103031
    Sample: Primary TGNs
  54. MOLECULES (2022) IF: 4.927
    Pharmacological Basis of Rumex hastatus D. Don in Gastrointestinal Diseases with Focusing Effects on H+/K+-ATPase, Calcium Channels Inhibition and PDE Mediated Signaling: Toxicological Evaluation on Vital Organs

    DOI: 10.3390/molecules27185919

    PMID: 36144661

    		 Sample: stomach tissue
  55. MOLECULES (2023) IF: 4.927
    Neuroprotective Effects of Savinin on LPS-Induced Neuroinflammation In Vivo via Regulating MAPK/NF-κB Pathway and NLRP3 Inflammasome Activation

    DOI: 10.3390/molecules28041575
    Sample: hippocampa
  56. MOLECULES (2022) IF: 4.927
    Modulatory Effect of Diosmin and Diosmetin on Metalloproteinase Activity and Inflammatory Mediators in Human Skin Fibroblasts Treated with Lipopolysaccharide

    DOI: 10.3390/molecules27134264

    PMID: 35807509

    		 Sample: human fibroblast cell
  57. Metabolites (2022) IF: 4.932
    Serum Metabolomics Based on GC-MS Reveals the Antipyretic Mechanism of Ellagic Acid in a Rat Model

    DOI: 10.3390/metabo12060479

    PMID: 35736412

    		 Sample: cerebrospinal fluid (CSF)
  58. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Gastroprotective effect of an active ingredients group of Lindera reflexa Hemsl. On Ethanol-Induced gastric ulcers in Rats: Involvement of VEGFR2/ERK and TLR-2/Myd88 signaling pathway

    DOI: 10.1016/j.intimp.2022.108673

    PMID: 35259712

    		 Sample: blood
  59. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Daurisoline alleviated experimental colitis in vivo and in vitro: Involvement of NF-κB and Wnt/β-Catenin pathway

    DOI: 10.1016/j.intimp.2022.108714

    PMID: 35366641

    		 Sample: colon tissues
  60. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2021) IF: 3
    Limonin modulated immune and inflammatory responses to suppress colorectal adenocarcinoma in mice model

    DOI: 10.1007/s00210-021-02101-6

    PMID: 34009457

    		 Sample: Serum
  61. JOURNAL OF ETHNOPHARMACOLOGY (2018) IF: 3.115
    Anti-inflammatory action of Athyrium multidentatum extract suppresses the LPS-induced TLR4 signaling pathway

    DOI: 10.1016/j.jep.2018.02.031
    Sample: peritoneal macrophages (PMs)
  62. REPRODUCTION (2019) IF: 3.125
    Endometrial stromal cell MiR-29c-3p microRNA regulates uterine contraction

    DOI: 10.1530/REP-19-0196
    Sample: ESCs cells
  63. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY (2019) IF: 3.144
    Cisplatin induces chemoresistance through the PTGS2-mediated anti-apoptosis in gastric cancer

    DOI: 10.1016/j.biocel.2019.105610

    PMID: 31518663

    		 Sample: Cell culture supernatant
  64. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023) IF: 3.195
    Pharmacological evaluation of carvacrol anti-migraine potential

    DOI: 10.1007/s00210-023-02412-w
    Sample: brain
  65. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2021) IF: 3.205
    A biolabel research based on metabonomics reveals the therapeutic potentials of Herba Lysimachiae in synovial diseases: The dual effects on synovial platelet aggregation by prostaglandin E1/E2

    DOI: 10.1016/j.jchromb.2021.122726

    PMID: 33957357

    		 Sample: Tissue homogenate(synovial)
  66. INFLAMMATION (2020) IF: 3.212
    Casticin Attenuates Osteoarthritis-Related Cartilage Degeneration by Inhibiting the ROS-Mediated NF-κB Signaling Pathway in vitro and in vivo

    DOI: 10.1007/s10753-019-01167-y
    Sample: serum,ADTC5 cell
  67. INFLAMMOPHARMACOLOGY (2020) IF: 3.238
    Polystichum braunii extracts inhibit Complete Freund’s adjuvant-induced arthritis via upregulation of I-κB, IL-4, and IL-10, downregulation of COX-2, PGE2, IL-1β, IL-6, NF-κB, and TNF-α, and subsiding oxidative stress

    DOI: 10.1007/s10787-020-00688-5
    Sample: serum
  68. Food & Function (2019) IF: 3.241
    A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese rats

    DOI: 10.1039/C9FO01342C
    Sample: serum
  69. NEUROUROLOGY AND URODYNAMICS (2018) IF: 3.263
    High luteinizing hormone weakens urinary continence mechanisms in association with prostaglandin E2 elevation in a postmenopausal rat model

    DOI: 10.1002/nau.23470
    Sample: serum
  70. INFLAMMOPHARMACOLOGY (2018) IF: 3.304
    prevented osteoarthritis cartilage degeneration by attenuating joint inflammation and collagen breakdown in postmenopausal rat model

    DOI: 10.1007/s10787-018-0452-6
    Sample: serum
  71. INFLAMMOPHARMACOLOGY (2018) IF: 3.304
    ) protected against osteoarthritis by mitigating inflammation and cartilage degradation: a preclinical study

    DOI: 10.1007/s10787-017-0432-2
    Sample: serum
  72. Processes (2022) IF: 3.352
    Gastroprotective, Biochemical, and Acute Toxicity Effects of Papaver decaisnei against Ethanol-Induced Gastric Ulcers in Rats

    DOI: 10.3390/pr10101985
    Sample: gastric tissue
  73. Processes (2022) IF: 3.352
    Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

    DOI: 10.3390/pr10071314
    Sample: stomach
  74. CHEMICO-BIOLOGICAL INTERACTIONS (2019) IF: 3.407
    Microglial activation and inflammation caused by traffic-related particulate matter

    DOI: 10.1016/j.cbi.2019.108762
    Sample: BV-2 cells
  75. Biomed Research International (2022) IF: 3.411
    Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model

    DOI: 10.1155/2022/4117520

    PMID: 35509713

    		 Sample: plasma
  76. TOXICOLOGY IN VITRO (2021) IF: 3.5
    (3α)-3-(tiglinoyloxy)-ent-kaur-16-en-19-oic acid, isolated from Wedelia trilobata L., exerts an anti-inflammatory effect via the modulation of NF-κB, MAPK and mTOR pathway and autophagy in LPS-stimulated macrophages

    DOI: 10.1016/j.tiv.2021.105139

    PMID: 33705895

    		 Sample: Cell culture supernatant
  77. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS (2020) IF: 3.561
    Microsomal Prostaglandin E2 Synthase-1 Deletion Attenuates Isoproterenol-Induced Myocardial Fibrosis in Mice

    DOI: 10.1124/jpet.120.000023
    Sample: Plasma
  78. PHYTOMEDICINE (2018) IF: 3.61
    Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways

    DOI: 10.1016/j.phymed.2018.11.015

    PMID: 31005814

    		 Sample: Cell culture supernatant
  79. LIFE SCIENCES (2020) IF: 3.647
    PGE2-HIF1α reciprocal induction regulates migration, phenotypic alteration and immunosuppressive capacity of macrophages in tumor microenvironment

    DOI: 10.1016/j.lfs.2020.117731
    Sample: macrophages
  80. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021) IF: 3.652
    Thymol ameliorates 5-fluorouracil-induced intestinal mucositis: Evidence of down-regulatory effect on TGF-β/MAPK pathways through NF-κB

    DOI: 10.1002/jbt.22932

    PMID: 34665902

    		 Sample: serum,intestianl tissue
  81. AMERICAN JOURNAL OF CHINESE MEDICINE (2020) IF: 3.682
    Corydalis saxicola Alkaloids Attenuate Cisplatin-Induced Neuropathic Pain by Reducing Loss of IENF and Blocking TRPV1 Activation

    DOI: 10.1142/S0192415X20500214

    PMID: 32138533

    		 Sample: Serum
  82. BIOLOGICAL TRACE ELEMENT RESEARCH (2021) IF: 3.738
    Effect of Telmisartan on Arsenic-Induced (Sub-chronic) Perturbations in Redox Homeostasis, Pro-inflammatory Cascade and Aortic Dysfunction in Wistar Rats

    DOI: 10.1007/s12011-021-02804-0

    PMID: 34339004

    		 Sample: aorta
  83. JOURNAL OF NATURAL PRODUCTS (2020) IF: 3.782
    3α-Angeloyloxy-ent-kaur-16-en-19-oic Acid Isolated from Wedelia trilobata L. Alleviates Xylene-Induced Mouse Ear Edema and Inhibits NF-κB and MAPK Pathway in LPS-Stimulated Macrophages

    DOI: 10.1021/acs.jnatprod.0c00990
    Sample: Cell culture medium,Tissue Slice
  84. INFLAMMOPHARMACOLOGY (2019) IF: 3.838
    leaf extracts attenuate Complete Freund’s adjuvant-induced arthritis in Wistar rats via modulation of inflammatory and oxidative stress biomarkers

    DOI: 10.1007/s10787-019-00596-3
    Sample: Serum
  85. Frontiers in Pharmacology (2019) IF: 3.845
    The Role of Prostaglandin-Endoperoxide Synthase-2 in Chemoresistance of Non-Small Cell Lung Cancer

    DOI: 10.3389/fphar.2019.00836

    PMID: 31440159

    		 Sample: Cell culture supernatant
  86. Annals of Translational Medicine (2021) IF: 3.932
    Dexamethasone-loaded thermo-sensitive hydrogel attenuates osteoarthritis by protecting cartilage and providing effective pain relief

    DOI: 10.21037/atm-21-684

    PMID: 34430561

    		 Sample: serum
  87. NEUROCHEMISTRY INTERNATIONAL (2019) IF: 3.994
    Phospho-mTOR expression in human glioblastoma microglia-macrophage cells

    DOI: 10.1016/j.neuint.2019.104485
    Sample: CHME-5 cells,RRID cells,CVCL_5J53 cells,U87MGcells
  88. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
    Metformin attenuated histopathological ocular deteriorations in a streptozotocin-induced hyperglycemic rat model

    DOI: 10.1007/s00210-020-01989-w

    PMID: 33047165

    		 Sample: Serum
  89. STEROIDS (2019) IF: 2.136
    Synthesis and evaluation of novel D-ring substituted steroidal pyrazolines as potential anti-inflammatory agents

    DOI: 10.1016/j.steroids.2019.03.012

    PMID: 30951758

    		 Sample: Cell culture supernatant
  90. International Journal of Peptide Research and Therapeutics (2022) IF: 2.191
    Atrial Natriuretic Peptide (ANP) Suppress λ-Carrageenan-Induced Acute Paw Edema by Modulating Pro-inflammatory Genes in Swiss Albino Mice

    DOI: 10.1007/s10989-022-10444-8
    Sample: paw tissue
  91. Biomed Research International (2019) IF: 2.197
    Galangin Suppresses Renal Inflammation via the Inhibition of NF-κB, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells

    DOI: 10.1155/2019/3018357

    PMID: 31240210

    		 Sample: Cell culture supernatant
  92. PROSTAGLANDINS & OTHER LIPID MEDIATORS (2020) IF: 2.283
    Effect of calcium hydroxide alone or in combination with ibuprofen and ciprofloxacin on postoperative pain and periapical prostaglandin E2 level: A randomized clinical study

    DOI: 10.1016/j.prostaglandins.2020.106525

    PMID: 33383182

    		 Sample: biological fluids
  93. BIOLOGICAL TRACE ELEMENT RESEARCH (2018) IF: 2.361
    Melatonin Ameliorates Neuropharmacological and Neurobiochemical Alterations Induced by Subchronic Exposure to Arsenic in Wistar Rats

    DOI: 10.1007/s12011-018-1537-1

    PMID: 30306420

    		 Sample: Tissue homogenate
  94. ACTA ASTRONAUTICA (2021) IF: 2.413
    Contribution of endoplasmic reticulum stress response to the mechanosensitivity alteration in osteocytes under simulated microgravity

    DOI: 10.1016/j.actaastro.2021.11.037
    Sample: MLO-Y4 cell
  95. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Vicenin 3 ameliorates ECM degradation by regulating the MAPK pathway in SW1353 chondrocytes

    DOI: 10.3892/etm.2021.10896
    Sample: SW1353 cell
  96. Experimental and Therapeutic Medicine (2022) IF: 2.447
    The complement C3a‑C3aR and C5a‑C5aR pathways promote viability and inflammation of human retinal pigment epithelium cells by targeting NF‑κB signaling

    DOI: 10.3892/etm.2022.11420
    Sample: HRPE cell
  97. Journal of Pain Research (2017) IF: 2.581
    Involvement of endoplasmic reticulum stress in formalin-induced pain is attenuated by 4-phenylbutyric acid

    DOI: 10.2147/JPR.S125805
    Sample: Cerebrospinal fluid
  98. BMC Pharmacology & Toxicology (2022) IF: 2.605
    Pharmacological evaluation of newly synthesized organotin IV complex for antiulcer potential

    DOI: 10.1186/s40360-022-00596-0

    PMID: 35906691

    		 Sample: Stomach tissue
  99. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    The Anti-Inflammatory Effect of Smilax china L. Extract on LPS-Stimulated THP-1 via Downregulation of MAPK and NF-κB Signaling Pathway

    DOI: 10.1155/2021/9958808
    Sample: THP-1 cells
  100. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Proteomic Analysis and In Vivo Studies Reveal the Potential Gastroprotective Effects of CHCl3 and Aqueous Extracts of Ficus palmata

    DOI: 10.1155/2021/6613140
    Sample: Tissue homogenate
  101. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Preclinical Rodent Models of Arthritis and Acute Inflammation Indicate Immunomodulatory and Anti-Inflammatory Properties of Juglans regia Extracts

    DOI: 10.1155/2022/1695701

    PMID: 35422870

    		 Sample: serum
  102. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    Anti-inflammatory Mechanism of Action of Benzoylmesaconine in Lipopolysaccharide-Stimulated RAW264.7 Cells

    DOI: 10.1155/2022/7008907

    PMID: 35873638

    		 Sample: RAW264.7 cell
  103. BIOMARKERS (2022) IF: 2.658
    Gastro-protective effect of Artemisia sieberi essential oil against ethanol-induced ulcer in rats as revealed via biochemical, histopathological and metabolomics analysis

    DOI: 10.1080/1354750X.2021.2025428

    PMID: 34978233

    		 Sample: stomach tissue
  104. BIOMARKERS (2022) IF: 2.663
    Metabolomics approach of Symphyotrichum squamatum ethanol extract and its nano-Ag formulation protective effect on gastric ulcer via bio-chemical and pathological analyses

    DOI: 10.1080/1354750X.2022.2157488
    Sample: stomach tissue
  105. STEROIDS (2021) IF: 2.668
    Synthesis and anti-inflammatory activity of novel steroidal chalcones with 3β-pregnenolone ester derivatives in RAW 264.7 cells in vitro

    DOI: 10.1016/j.steroids.2021.108830

    PMID: 33836205

    		 Sample: Cell culture supernatant
  106. Iranian Journal of Basic Medical Sciences (2021) IF: 2.699
    Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)

    DOI: 10.22038/IJBMS.2021.59348.13174
    Sample: RAW 264.7 cell
  107. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    Gastroprotective effect of hydromethanolic extract of Ayapana triplinervis leaves on indomethacin-induced gastric ulcer in male Wistar rats

    DOI: 10.1111/jfbc.13859

    PMID: 34258791

    		 Sample: serum
  108. MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY (2017) IF: 2.733
    Mistletoe fig (Ficus deltoidea Jack) leaf extract prevented postmenopausal osteoarthritis by attenuating inflammation and cartilage degradation in rat model

    DOI: 10.1097/GME.0000000000000882
    Sample: serum
  109. Pharmacological Reports (2020) IF: 2.754
    Differential effects of performance-enhancing drugs ‘Methamphetamine’ and ‘hCG’ on ex-vivo cultured primary blood mononuclear cells of male athletes

    DOI: 10.1007/s43440-020-00066-6
    Sample: Cell culture supernatant
  110. Plants-Basel (2020) IF: 2.762
    Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

    DOI: 10.3390/plants9091127

    PMID: 32878164

    		 Sample: Cell culture supernatant
  111. Journal of Physiological Sciences (2022) IF: 2.781
    MiR-18a-3p improves cartilage matrix remodeling and inhibits inflammation in osteoarthritis by suppressing PDP1

    DOI: 10.1186/s12576-022-00827-3

    PMID: 35148687

    		 Sample: serum
  112. Medicina-Lithuania (2022) IF: 2.948
    Pretreatment of Garlic Oil Extracts Hampers Epithelial Damage in Cell Culture Model of Peptic Ulcer Disease

    DOI: 10.3390/medicina58010091
    Sample: AGS cell
  113. Molecular Medicine Reports (2021) IF: 2.952
    α‑rhamnrtin‑3‑α‑rhamnoside exerts anti‑inflammatory effects on lipopolysaccharide‑stimulated RAW264.7 cells by abrogating NF‑κB and activating the Nrf2 signaling pathway

    DOI: 10.3892/mmr.2021.12439

    PMID: 34523697

    		 Sample: RAW264.7 cell
  114. Molecular & Cellular Toxicology (2021) IF: 1.08
    Fatty acid-binding protein 5 activates cyclooxygenase-2 and promotes hypoxic injury in LO2 cells

    DOI: 10.1007/s13273-021-00158-1
    Sample: LO2 cell
  115. EXPERIMENTAL ANIMALS (2018) IF: 1.173
    Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats

    DOI: 10.1538/expanim.18-0029
    Sample: Serum
  116. RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY (2022) IF: 1.254
    Anti-Inflammatory Effects of Coumarin–Selenophene Derivatives on LPS-Stimulated RAW 264.7 Macrophage Cells

    DOI: 10.1134/S1068162022060279
    Sample: RAW 264.7 cells
  117. NEUROREPORT (2018) IF: 1.266
    Gentiopicroside protects neurons from astrocyte-mediated inflammatory injuries by inhibition of nuclear factor-κB and mitogen-activated protein kinase signaling pathways

    DOI: 10.1097/WNR.0000000000001082

    PMID: 29958245

    		 Sample: Cell culture supernatant
  118. Journal of BUON (2019) IF: 1.379
    Effects of postoperative enteral nutrition combined with adjuvant radiotherapy on inflammatory response, nutrition, healing and prognosis in patients receiving radical surgery for esophageal carcinoma.

    PMID: 31646824

    		 Sample: serum
  119. MEDICAL SCIENCE MONITOR (2017) IF: 1.585
    Cross-Coupling Effects of Silencing of Cyclooxygenase-2 (COX-2)/Aggrecanase-1 and Over-Expressed Insulin-Like Growth Factor 1 (IGF-1) in an Osteoarthritis Animal Model

    DOI: 10.12659/MSM.907150
    Sample: synovial fluid
  120. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    Citrus hystrix leaf extract attenuated diabetic‐cataract in STZ‐rats

    DOI: 10.1111/jfbc.13258
    Sample: serum
  121. Evidence-based Complementary and Alternative Medicine (2020) IF: 1.813
    Evaluation of Immunomodulatory and Antiarthritic Potential of Trigonella gharuensis Extracts

    DOI: 10.1155/2020/8836080

    PMID: 33381210

    		 Sample: Serum
  122. Molecular Medicine Reports (2019) IF: 1.851
    MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways

    DOI: 10.3892/mmr.2019.10269
    Sample: serum
  123. Journal of Otolaryngology-Head & Neck Surgery (2020) IF: 1.852
    Simple hypertrophic tonsils have more active innate immune and inflammatory responses than hypertrophic tonsils with recurrent inflammation in children

    DOI: 10.1186/s40463-020-00428-3

    PMID: 32487224

    		 Sample: Tissue homogenate
  124. MEDICINE (2019) IF: 1.87
    Role of ascitic prostaglandin E2 in diagnosis of spontaneous bacterial peritonitis and prediction of in-hospital mortality in patients with decompensated cirrhosis

    DOI: 10.1097/MD.0000000000016016

    PMID: 31261505

    		 Sample: Ascitic fluid
  125. STEROIDS (2020) IF: 1.948
    Russelioside B; A pregnane glycoside for treatment of gastric ulcer via modulation of heat shock protein-70 and vascular endothelial growth factor

    DOI: 10.1016/j.steroids.2020.108759

    PMID: 33181144

    		 Sample: Tissue homogenate
  126. Journal of Diabetes and Metabolic Disorders (2022)
    Protective effects of Labisia pumila against neuropathy in a diabetic rat model

    DOI: 10.1007/s40200-021-00905-0
    Sample: serum
  127. Oriental Pharmacy and Experimental Medicine (2019)
    Prostaglandin E2 and oxidative defense system contributed to anti-nociception action of aqueous Adansonia digitata bark extract in induced neuropathic pain in Wistar rats

    DOI: 10.1007/s13596-019-00373-1
    Sample: plasma
  128. Beni-Suef University Journal of Basic and Applied Sciences (2022)
    Molecular docking prediction and in vitro studies elucidate anti-inflammatory effect of Garcinia extract against inducible nitric oxide synthase and cyclooxygenase-2 targets

    DOI: 10.1186/s43088-022-00214-2
    Sample: RAW264.7 cell
  129. SCIENTIA PHARMACEUTICA (2022)
    Colorectal Cancer Chemoprevention by S-Allyl Cysteine–Caffeic Acid Hybrids: In Vitro Biological Activity and In Silico Studies

    DOI: 10.3390/scipharm90030040
    Sample: SW480 cell
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L**************nSubmitted [ Mar 08 2022 ]

Asked: Hi there! I need PGE2 elisa kit that capable to detect PGE2 from RAW and Caco2 cell. is your product (E-EL-0034) capable to detect from mouse and human cell line? Hope to see your response soon. Thank u

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A

adminSubmitted [ Mar 08 2022 ]

Answered: Dear Setiawan, GREETINGS FROM ELABSCIENCE! E-EL-0034 PGE2(Prostaglandin E2) ELISA Kit It can bes used to detect PGE2 from RAW and Caco2 from mouse and human cell line. Any questions pls feel free to ask ya~ It's my email address: Amanda@elabscience.com

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