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Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

Citations(30)

  • Cat.No.:E-EL-R1327

  • Reactivity: Rat
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat CKMB in samples. No significant cross-reactivity or interference between Rat CKMB and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat CKMB concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CKMB. You can calculate the concentration of Rat CKMB in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CKMB were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CKMB were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 93.35 286.64 760.84 100.16 266.78 728.82
Standard deviation 4.85 13.27 36.44 6.24 15.71 23.91
CV (%) 5.20 4.63 4.79 6.23 5.89 3.28

Recovery

The recovery of Rat CKMB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 93
EDTA plasma (n=8) 90-105 98
Cell culture media (n=8) 87-99 93

Linearity

Samples were spiked with high concentrations of Rat CKMB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCKMB
Research AreaCardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Nature Communications (2021) IF: 14.919
    The long noncoding RNA lncCIRBIL disrupts the nuclear translocation of Bclaf1 alleviating cardiac ischemia–reperfusion injury

    DOI: 10.1038/s41467-020-20844-3

    PMID: 33483496

    		 Sample: Plasma
  2. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
    Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

    DOI: 10.1186/s12951-022-01639-8

    PMID: 36195952

    		 Sample: blood
  3. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Cardioprotective Effects of Aconite in Isoproterenol-Induced Myocardial Infarction in Rats

    DOI: 10.1155/2022/1090893
    Sample: serum
  4. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Chaihujialonggumulitang shows psycho-cardiology therapeutic effect on acute myocardial infarction with comorbid anxiety by the activation of Nrf2/HO-1 pathway and suppression of oxidative stress and apoptosis

    DOI: 10.1016/j.biopha.2022.113437

    PMID: 36076489

    		 Sample: serum
  5. LIFE SCIENCES (2022) IF: 6.78
    Astragaloside IV ameliorates sepsis-induced myocardial dysfunction by regulating NOX4/JNK/BAX pathway

    DOI: 10.1016/j.lfs.2022.121123

    PMID: 36302499

    		 Sample: myocardium,serum
  6. CLINICAL SCIENCE (2016) IF: 5.016
    Selective inhibition of PTEN preserves ischaemic post-conditioning cardioprotection in STZ-induced Type 1 diabetic rats: role of the PI3K/Akt and JAK2/STAT3 pathways

    DOI: 10.1042/CS20150496
    Sample: Serum
  7. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 5.195
    A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation

    DOI: 10.1016/j.ejphar.2022.175172

    PMID: 35944619

    		 Sample: serum
  8. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
    microRNA-454-mediated NEDD4-2/TrkA/cAMP axis in heart failure: Mechanisms and cardioprotective implications

    DOI: 10.1111/jcmm.16491

    PMID: 33949117

    		 Sample: Plasma
  9. Journal of the American Heart Association (2022) IF: 5.501
    Trimetazidine Alleviates Postresuscitation Myocardial Dysfunction and Improves 96‐Hour Survival in a Ventricular Fibrillation Rat Model

    DOI: 10.1161/JAHA.121.023378
    Sample: plasma
  10. Frontiers in Cardiovascular Medicine (2022) IF: 5.846
    Adenosine A2a Receptor Regulates Autophagy Flux and Apoptosis to Alleviate Ischemia-Reperfusion Injury via the cAMP/PKA Signaling Pathway

    DOI: 10.3389/fcvm.2022.755619
    Sample: cardiomyocytes
  11. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats

    DOI: 10.1007/s11356-021-15409-w

    PMID: 34322808

    		 Sample: serum
  12. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Glycyrrhizic acid ameliorates myocardial ischemia-reperfusion injury in rats through inhibiting endoplasmic reticulum stress

    DOI: 10.1016/j.ejphar.2021.174353

    PMID: 34274339

    		 Sample: serum
  13. Cardiovascular Diabetology (2016) IF: 4.534
    N-acetylcysteine attenuates myocardial dysfunction and postischemic injury by restoring caveolin-3/eNOS signaling in diabetic rats

    DOI: 10.1186/s12933-016-0460-z
    Sample: Serum
  14. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats.

    DOI: 10.26355/eurrev_202006_21528
    Sample: Serum
  15. RSC Advances (2019) IF: 3.049
    Morroniside alleviates coxsackievirus B3-induced myocardial damage apoptosis via restraining NLRP3 inflammasome activation

    DOI: 10.1039/C8RA08662A
    Sample: Tissue homogenate
  16. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Tongmai Yangxin pills anti-oxidative stress alleviates cisplatin-induced cardiotoxicity: Network pharmacology analysis and experimental evidence

    DOI: 10.1016/j.biopha.2018.09.095
    Sample: serum
  17. Annals of Translational Medicine (2022) IF: 3.616
    Activation of AMPK/p38/Nrf2 is involved in resveratrol alleviating myocardial ischemia-reperfusion injury in diabetic rats as an endogenous antioxidant stress feedback

    DOI: 10.21037/atm-22-3789

    PMID: 36111006

    		 Sample: serum
  18. IMMUNOLOGICAL INVESTIGATIONS (2020) IF: 3.657
    Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase‑1/IL‑1β Signaling Pathway Inhibition

    DOI: 10.1080/08820139.2020.1791178
    Sample: Cell culture supernatant
  19. BRITISH JOURNAL OF NUTRITION (2022) IF: 3.718
    Evidence based mechanistic role of Chrysin towards protection of cardiac hypertrophy & fibrosis in rats

    DOI: 10.1017/S0007114522000472
    Sample: serum
  20. BIOSCIENCE REPORTS (2022) IF: 3.976
    Role of Keap1-Nrf2/ARE signal transduction pathway in protection of dexmedetomidine preconditioning against myocardial ischemia/reperfusion injury

    DOI: 10.1042/BSR20221306

    PMID: 35959640

    		 Sample: serum
  21. Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2022) IF: 2.01
    Carnosic Acid Protects Against Myocardial Infarction by Controlling Oxidative Stress and Inflammation in Rats

    DOI: 10.1007/s43450-021-00216-8
    Sample: serum
  22. XENOBIOTICA (2015) IF: 2.199
    Protective effects of berberine against doxorubicin-induced cardiotoxicity in rats by inhibiting metabolism of doxorubicin

    DOI: 10.3109/00498254.2015.1034223
    Sample: Serum
  23. JOURNAL OF PHARMACY AND PHARMACOLOGY (2018) IF: 2.309
    Total flavones of Rhododendron simsii Planch flower protect isolated rat heart from ischaemia‐reperfusion injury and its mechanism of UTR‐RhoA‐ROCK pathway inhibition

    DOI: 10.1111/jphp.13016
    Sample: Tissue homogenate
  24. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Dynamic observation of 5‑fluorouracil‑induced myocardial injury and mitochondrial autophagy in aging rats

    DOI: 10.3892/etm.2021.10886
    Sample: serum
  25. Pharmacological Reports (2019) IF: 2.761
    Bisoprolol and linagliptin ameliorated electrical and mechanical isometric myocardial contractions in doxorubicin-induced cardiomyopathy in rats

    DOI: 10.1007/s43440-019-00034-9
    Sample: plasma
  26. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019) IF: 2.965
    Hesperidin regresses cardiac hypertrophy by virtue of PPAR‐γ agonistic, anti‐inflammatory, antiapoptotic, and antioxidant properties

    DOI: 10.1002/jbt.22283
    Sample: Serum
  27. PHARMACEUTICAL BIOLOGY (2020) IF: 2.971
    Effect of phenylacetamide isolated from lepidium apetalum on myocardial injury in spontaneously hypertensive rats and its possible mechanism

    DOI: 10.1080/13880209.2020.1778043
    Sample: serum
  28. Molecular Medicine Reports (2015) IF: 1.554
    Candesartan ameliorates acute myocardial infarction in rats through inducible nitric oxide synthase, nuclear factor‑κB, monocyte chemoattractant protein‑1, activator protein‑1 and restoration of heat shock protein 72

    DOI: 10.3892/mmr.2015.4432
    Sample: Serum
  29. KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY (2019) IF: 1.654
    Effects of heme oxygenase-1 upregulation on isoproterenol-induced myocardial infarction

    DOI: 10.4196/kjpp.2019.23.3.203
    Sample: serum
  30. Molecular Medicine Reports (2017) IF: 1.692
    AMPK activation restores ischemic post‑conditioning cardioprotection in STZ‑induced type 1 diabetic rats: Role of autophagy

    DOI: 10.3892/mmr.2017.7033
    Sample: Serum
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