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Rat TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit

Cat.No.:E-EL-R1253
Reactivity: Rat

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Rat TNNI3/cTn-I and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNNI3/cTn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNNI3/cTn-I. You can calculate the concentration of Rat TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TNNI3/cTn-I were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TNNI3/cTn-I were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	102.40	248.30	900.40	97.00	237.90	894.70
Standard deviation	5.70	14.90	36.90	5.10	11.40	37.60
CV (%)	5.57	6.00	4.10	5.26	4.79	4.20

Recovery

The recovery of Rat TNNI3/cTn-I spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-110	101
EDTA plasma (n=8)	93-111	101
Cell culture media (n=8)	93-107	100

Linearity

Samples were spiked with high concentrations of Rat TNNI3/cTn-I and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	TNNC1, cTnI, CMD1FF, CMD2A, CMH7, RCM1
Research Area	Cancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMATERIALS (2021) IF: 12.479
Polyphenol-assisted facile assembly of bioactive nanoparticles for targeted therapy of heart diseases

DOI: 10.1016/j.biomaterials.2021.120952

PMID: 34147720
Sample: serum
JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

DOI: 10.1186/s12951-022-01639-8

PMID: 36195952
Sample: blood
Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
Cardioprotective Effects of Aconite in Isoproterenol-Induced Myocardial Infarction in Rats

DOI: 10.1155/2022/1090893

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
Investigation of the impact of rosuvastatin and telmisartan in doxorubicin-induced acute cardiotoxicity

DOI: 10.1016/j.biopha.2022.113673

Sample: serum
LIFE SCIENCES (2022) IF: 6.78
Astragaloside IV ameliorates sepsis-induced myocardial dysfunction by regulating NOX4/JNK/BAX pathway

DOI: 10.1016/j.lfs.2022.121123

PMID: 36302499
Sample: myocardium,serum
PHYTOMEDICINE (2021) IF: 5.34
Cardioprotective activity of ethyl acetate extract of Cinnamomi Ramulus against myocardial ischemia/reperfusion injury in rats via inhibiting NLRP3 inflammasome activation and pyroptosis

DOI: 10.1016/j.phymed.2021.153798

PMID: 34673348
Sample: serum
Journal of the American Heart Association (2021) IF: 5.501
Post‐Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest

DOI: 10.1161/JAHA.120.019837

Sample: Plasma
REDOX REPORT (2023) IF: 5.696
Dexmedetomidine postconditioning attenuates myocardial ischemia/reperfusion injury by activating the Nrf2/Sirt3/SOD2 signaling pathway in the rats

DOI: 10.1080/13510002.2022.2158526

Sample: Serum
Frontiers in Cardiovascular Medicine (2022) IF: 5.846
Adenosine A2a Receptor Regulates Autophagy Flux and Apoptosis to Alleviate Ischemia-Reperfusion Injury via the cAMP/PKA Signaling Pathway

DOI: 10.3389/fcvm.2022.755619

Sample: cardiomyocytes
ACTA PHARMACOLOGICA SINICA (2019) IF: 4.01
Exogenous NADPH ameliorates myocardial ischemia–reperfusion injury in rats through activating AMPK/mTOR pathway

DOI: 10.1038/s41401-019-0301-1

Sample: Serum
INFLAMMATION (2021) IF: 4.092
MiR-708-3p Alleviates Inflammation and Myocardial Injury After Myocardial Infarction by Suppressing ADAM17 Expression

DOI: 10.1007/s10753-020-01404-9

PMID: 33492555
Sample: Cell culture supernatant
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats

DOI: 10.1007/s11356-021-15409-w

PMID: 34322808
Sample: serum
SAUDI PHARMACEUTICAL JOURNAL (2021) IF: 4.33
Cinnamomum zeylanicum Blume (Ceylon cinnamon) bark extract attenuates doxorubicin induced cardiotoxicity in Wistar rats

DOI: 10.1016/j.jsps.2021.06.004

PMID: 34408544
Sample: serum
EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 4.432
Piperazine ferulate protects against cardiac ischemia/reperfusion injury in rat via the suppression of NLRP3 inflammasome activation and pyroptosis

DOI: 10.1016/j.ejphar.2022.174856

PMID: 35219728
Sample: Myocardial
MOLECULES (2022) IF: 4.927
7-Hydroxyflavone Alleviates Myocardial Ischemia/Reperfusion Injury in Rats by Regulating Inflammation

DOI: 10.3390/molecules27175371

PMID: 36080137
Sample: serum
European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats.

DOI: 10.26355/eurrev_202006_21528

Sample: Serum
JOURNAL OF CELLULAR BIOCHEMISTRY (2017) IF: 3.085
Functional and Structural Assessment of the Effect of Human Umbilical Cord Blood Mesenchymal Stem Cells in Doxorubicin‐Induced Cardiotoxicity

DOI: 10.1002/jcb.26168

Sample: Serum
PLoS One (2015) IF: 3.24
The Protective Role of Interleukin-33 in Myocardial Ischemia and Reperfusion Is Associated with Decreased HMGB1 Expression and Up-Regulation of the P38 MAPK Signaling Pathway

DOI: 10.1371/journal.pone.0143064

Sample: Serum,Tissue homogenate
Life-Basel (2022) IF: 3.251
Protective Effects of L-2-Oxothiazolidine-4-Carboxylate during Isoproterenol-Induced Myocardial Infarction in Rats: In Vivo Study

DOI: 10.3390/life12101466

PMID: 36294901
Sample: plasma
EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263
Carbon monoxide attenuates LPS-induced myocardial dysfunction in rats by regulating the mitochondrial dynamic equilibrium

DOI: 10.1016/j.ejphar.2020.173726

PMID: 33159931
Sample: Serum
Bioengineered (2022) IF: 3.269
Clemastine protects against sepsis-induced myocardial injury in vivo and in vitro

DOI: 10.1080/21655979.2022.2047256

PMID: 35274595
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
Tongmai Yangxin pills anti-oxidative stress alleviates cisplatin-induced cardiotoxicity: Network pharmacology analysis and experimental evidence

DOI: 10.1016/j.biopha.2018.09.095

Sample: serum
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575
Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

DOI: 10.1016/j.bbrc.2018.02.121

Sample: serum
IMMUNOLOGICAL INVESTIGATIONS (2020) IF: 3.657
Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase‑1/IL‑1β Signaling Pathway Inhibition

DOI: 10.1080/08820139.2020.1791178

Sample: Cell culture supernatant
INFLAMMOPHARMACOLOGY (2019) IF: 3.838
Urotensin-#receptor antagonist SB-706375 protected isolated rat heart from ischaemia–reperfusion injury by attenuating myocardial necrosis via RhoA/ROCK/RIP3 signalling pathway

DOI: 10.1007/s10787-019-00598-1

Sample: Bronchoalveolar Lavage Fluid
BIOSCIENCE REPORTS (2022) IF: 3.976
Role of Keap1-Nrf2/ARE signal transduction pathway in protection of dexmedetomidine preconditioning against myocardial ischemia/reperfusion injury

DOI: 10.1042/BSR20221306

PMID: 35959640
Sample: serum
BMC Cardiovascular Disorders (2022) IF: 2.174
Assessing the risk factors for myocardial infarction in diet-induced prediabetes: myocardial tissue changes

DOI: 10.1186/s12872-022-02758-8

PMID: 35918636
Sample: heart tissue
Experimental and Therapeutic Medicine (2019) IF: 2.447
H2S attenuates sepsis‑induced cardiac dysfunction via a PI3K/Akt‑dependent mechanism

DOI: 10.3892/etm.2019.7440

Sample: serum
Experimental and Therapeutic Medicine (2021) IF: 2.447
Dynamic observation of 5‑fluorouracil‑induced myocardial injury and mitochondrial autophagy in aging rats

DOI: 10.3892/etm.2021.10886

Sample: serum
Chinese Journal of Integrative Medicine (2022) IF: 2.626
Shenmai Injection Attenuates Myocardial Ischemia/Reperfusion Injury by Targeting Nrf2/GPX4 Signalling-Mediated Ferroptosis

DOI: 10.1007/s11655-022-3620-x

PMID: 35997859
Sample: blood
Pharmacological Reports (2019) IF: 2.761
Bisoprolol and linagliptin ameliorated electrical and mechanical isometric myocardial contractions in doxorubicin-induced cardiomyopathy in rats

DOI: 10.1007/s43440-019-00034-9

Sample: plasma
SCANDINAVIAN CARDIOVASCULAR JOURNAL (2020) IF: 1.084
St. Thomas and del Nido cardioplegia are superior to Custodiol cardioplegia in a rat model of donor heart

DOI: 10.1080/14017431.2020.1846772

PMID: 33185130
Sample: Tissue homogenate
Emergency Medicine International (2015) IF: 1.112
New Insights into the Role of Metformin Effects on Serum Omentin-1 Levels in Acute Myocardial Infarction: Cross-Sectional Study

DOI: 10.1155/2015/283021

Sample: serum
MEDICAL SCIENCE MONITOR (2020) IF: 1.918
Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

DOI: 10.12659/MSM.919054

PMID: 31992687
Sample: Plasma
International Journal of Clinical and Experimental Pathology (2020) IF: 0.252
Sepsis causes heart injury through endoplasmic reticulum stress-mediated apoptosis signaling pathway

PMID: 32509067
Sample: serum

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DOI: 10.1016/j.biomaterials.2021.120952
PMID: 34147720	Sample: serum

DOI: 10.1186/s12951-022-01639-8
PMID: 36195952	Sample: blood

DOI: 10.1016/j.lfs.2022.121123
PMID: 36302499	Sample: myocardium,serum

DOI: 10.1016/j.phymed.2021.153798
PMID: 34673348	Sample: serum

DOI: 10.1007/s10753-020-01404-9
PMID: 33492555	Sample: Cell culture supernatant

DOI: 10.1007/s11356-021-15409-w
PMID: 34322808	Sample: serum

DOI: 10.1016/j.jsps.2021.06.004
PMID: 34408544	Sample: serum

DOI: 10.1016/j.ejphar.2022.174856
PMID: 35219728	Sample: Myocardial

DOI: 10.3390/molecules27175371
PMID: 36080137	Sample: serum

DOI: 10.1371/journal.pone.0143064
	Sample: Serum,Tissue homogenate

DOI: 10.1161/JAHA.120.019837
	Sample: Plasma

DOI: 10.1080/13510002.2022.2158526
	Sample: Serum

DOI: 10.3389/fcvm.2022.755619
	Sample: cardiomyocytes

DOI: 10.1038/s41401-019-0301-1
	Sample: Serum

DOI: 10.3390/life12101466
PMID: 36294901	Sample: plasma

DOI: 10.1016/j.ejphar.2020.173726
PMID: 33159931	Sample: Serum

DOI: 10.1080/21655979.2022.2047256
PMID: 35274595	Sample: serum

DOI: 10.1080/08820139.2020.1791178
	Sample: Cell culture supernatant

DOI: 10.1007/s10787-019-00598-1
	Sample: Bronchoalveolar Lavage Fluid

DOI: 10.1042/BSR20221306
PMID: 35959640	Sample: serum

DOI: 10.1186/s12872-022-02758-8
PMID: 35918636	Sample: heart tissue

DOI: 10.1007/s11655-022-3620-x
PMID: 35997859	Sample: blood

DOI: 10.1080/14017431.2020.1846772
PMID: 33185130	Sample: Tissue homogenate

DOI: 10.12659/MSM.919054
PMID: 31992687	Sample: Plasma

Rat TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit

To Purchase E-EL-R1253

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Linearity

Target Information

Assay Procedures

Citations

BIOMATERIALS (2021) IF: 12.479

JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429

Oxidative Medicine and Cellular Longevity (2022) IF: 7.31

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419

LIFE SCIENCES (2022) IF: 6.78

PHYTOMEDICINE (2021) IF: 5.34

Journal of the American Heart Association (2021) IF: 5.501

REDOX REPORT (2023) IF: 5.696

Frontiers in Cardiovascular Medicine (2022) IF: 5.846

ACTA PHARMACOLOGICA SINICA (2019) IF: 4.01

INFLAMMATION (2021) IF: 4.092

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223

SAUDI PHARMACEUTICAL JOURNAL (2021) IF: 4.33

EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 4.432

MOLECULES (2022) IF: 4.927

European Review for Medical and Pharmacological Sciences (2020) IF: 3.024

JOURNAL OF CELLULAR BIOCHEMISTRY (2017) IF: 3.085

PLoS One (2015) IF: 3.24

Life-Basel (2022) IF: 3.251

EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263

Bioengineered (2022) IF: 3.269

BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575

IMMUNOLOGICAL INVESTIGATIONS (2020) IF: 3.657

INFLAMMOPHARMACOLOGY (2019) IF: 3.838

BIOSCIENCE REPORTS (2022) IF: 3.976

BMC Cardiovascular Disorders (2022) IF: 2.174

Experimental and Therapeutic Medicine (2019) IF: 2.447

Experimental and Therapeutic Medicine (2021) IF: 2.447

Chinese Journal of Integrative Medicine (2022) IF: 2.626

Pharmacological Reports (2019) IF: 2.761

SCANDINAVIAN CARDIOVASCULAR JOURNAL (2020) IF: 1.084

Emergency Medicine International (2015) IF: 1.112

MEDICAL SCIENCE MONITOR (2020) IF: 1.918

International Journal of Clinical and Experimental Pathology (2020) IF: 0.252

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