Recombinant Mouse β-NGF/NGFB Protein
Price: $ 739
Price: $ 249
Size:
100μg
20μg
Quantity:
- Expression Host: E.coli
- Accession: P01139
- Applications: Cell culture
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Product Details
Synonyms | Beta-nerve growth factor;Beta-NGF;Ngf |
Species | Mouse |
Expression Host | E.coli |
Sequence | Ser122-Gly241 |
Accession | P01139 |
Calculated Molecular Weight | 14.4 kDa |
Observed Molecular Weight | 11-17 kDa |
Tag | C-His |
Bio-activity | Measure by its ability to induce TF-1 cells proliferation. The ED<sub>50</sub> for this effect is <1ng/mL. The specific activity of recombinant mouse beta-NGF is > 1 x 10<sup>6</sup> IU/mg. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. |