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Recombinant Mouse β-NGF/NGFB Protein

Cat:PKSM041189
Manual MSDS

Price: $ 739

Price: $ 249

Size:
100μg 20μg
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  • Expression Host: E.coli
  • Accession: P01139
  • Applications: Cell culture
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Product Details
Synonyms Beta-nerve growth factor;Beta-NGF;Ngf
Species Mouse
Expression Host E.coli
Sequence Ser122-Gly241
Accession P01139
Calculated Molecular Weight 14.4 kDa
Observed Molecular Weight 11-17 kDa
Tag C-His
Bio-activity Measure by its ability to induce TF-1 cells proliferation. The ED<sub>50</sub> for this effect is <1ng/mL. The specific activity of recombinant mouse beta-NGF is > 1 x 10<sup>6</sup> IU/mg.
Purity > 98 % as determined by reducing SDS-PAGE.
Endotoxin < 0.1 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.