Renca [RenCa] Cell Line

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      Catalog number:EP-CL-0568

      Size:
      • 1×10⁶ cells/vial
      Qty:
      - +
      Price: $401

      Lead Time: 3-5 daysWelcome to order from local distributors.

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      Origin and General Characteristics

      Cell Name Renca [RenCa] Cell Line
      Age 6 weeks
      Tissue Kidney
      Morphology Epithelial-like
      Growth Properties Adherent
      Descriptions The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver. The cells do not express transforming growth factor-beta type II receptor (TbetaR-II).
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium RPMI-1640 +0.1mM NEAA +1mM Sodium Pyruvate +2mM L-glutamine +10% FBS +1% P/S
      Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
      Subcultivation Ratio 1:2-1:4
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
      Storage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%
      Temperature: 37℃

      Special Features of the Cell Line

      Tumorigenic Yes

      Related Resources

      Recommendations for handling of cryopreserved cells

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