SDHA Polyclonal Antibody
- +1
Price: $ 380
Price: $ 230
Price: $ 125
Price: $ 65
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:Mouse brain,MCF-7,Hep G2,Hela,Mouse brain,Mouse kidney,Rat brain,Rat kidney Verified Samples in IHC:Human liver,Human kidney |
Dilution |
WB 1:500-1:1000, IHC 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant Mouse EpCAM protein expressed by E.coli |
Abbre | SDHA |
Synonyms | CMD1GG;DHSA_HUMAN;Flavoprotein subunit of complex II;Fp;PGL5;SDH 2;SDH1;SDHF;Succinate dehydrogenase [ubiquinone] flavoprotein subunit |
Swissprot | |
Calculated MW | 73 kDa |
Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Mitochondrion inner membrane |
Concentration | 2.2mg/mL |
Buffer | PBS with 0.05% Proclin300 and 50% glycerol, pH7.4. |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology; Metabolism; Signal Transduction; Tags and Cell Markers |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. |
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Untargeted metabolomics and lipidomics analysis identified the role of FOXA1 in remodeling the metabolic pattern of BaP-transformed 16HBE cells
IF:4.219
Journal:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2021.115640
PMID:34242566