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SDHA Polyclonal Antibody

Cat:E-AB-40195 Citations (1)
Manual MSDS
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Price: $ 380

Price: $ 230

Price: $ 125

Price: $ 65

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Mouse brain,MCF-7,Hep G2,Hela,Mouse brain,Mouse kidney,Rat brain,Rat kidney
Verified Samples in IHC:Human liver,Human kidney
Dilution

WB 1:500-1:1000, IHC 1:50-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant Mouse EpCAM protein expressed by E.coli
Abbre SDHA
Synonyms CMD1GG;DHSA_HUMAN;Flavoprotein subunit of complex II;Fp;PGL5;SDH 2;SDH1;SDHF;Succinate dehydrogenase [ubiquinone] flavoprotein subunit
Swissprot
Calculated MW 73 kDa
Observed MW 70 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Mitochondrion inner membrane
Concentration 2.2mg/mL
Buffer PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
Purification Method Affinity purification
Research Areas Cancer; Cell Biology; Metabolism; Signal Transduction; Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.