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Tags and Controls Antibody

With the development of recombinant DNA technology, scientific researchers can construct recombinant fusion proteins which express target gene and have an epitope tag. Therefore, epitope tags are frequently used for identification and purification of recombinant proteins. Epitope tag antibodies are highly specific monoclonal/polyclonal antibodies with commonly used dye and enzyme conjugates and can serve as a universal detection method in Western blot.

An isotype control is the negative control in Flow cytometry and Immunohistochemistry, which is used to differentiate the non-specific background signal from specific antibody signal. The choice of isotype control antibody should combine the primary antibody’s host species, isotype and conjugation. 

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