4-HNE(4-Hydroxynonenal) ELISA Kit

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 4-HNE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 4-HNE were tested on 3 different plates, 20 replicates in each plate.

The recovery of 4-HNE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.