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Human OC/BGP(Osteocalcin) ELISA Kit

Citations(36)Uniprot : P02818

  • Cat.No.:E-EL-H1343

  • Reactivity: Human
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.25-80 ng/mL
Sensitivity 0.75 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human OC/BGP in samples. No significant cross-reactivity or interference between Human OC/BGP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human OC/BGP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OC/BGP. You can calculate the concentration of Human OC/BGP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human OC/BGP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human OC/BGP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 3.70 9.20 32.90 3.80 9.50 36.10
Standard deviation 0.20 0.40 1.60 0.30 0.50 1.30
CV (%) 5.41 4.35 4.86 7.89 5.26 3.60

Recovery

The recovery of Human OC/BGP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-109 99
EDTA plasma (n=8) 93-109 99
Cell culture media (n=8) 85-97 90

Linearity

Samples were spiked with high concentrations of Human OC/BGP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P02818
SynonymsBGLAP, OT, Bone Gamma-carboxyglutamate Protein, Bone Gla Protein
Research AreaCancer, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Materials Science & Engineering C-Materials for Biological Applications (2021) IF: 7.328
    Development of Si doped nano hydroxyapatite reinforced bilayer chitosan nanocomposite barrier membranes for guided bone regeneration

    DOI: 10.1016/j.msec.2021.112298

    PMID: 34474849

    		 Sample: Saos-2 cells
  2. Materials Science & Engineering C-Materials for Biological Applications (2021) IF: 7.328
    Bio-inspired multifunctional collagen/electrospun bioactive glass membranes for bone tissue engineering applications

    DOI: 10.1016/j.msec.2020.111856

    PMID: 34082925

    		 Sample: MG63 cells
  3. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
    Maternal omega-3 LC-PUFA supplementation programs an improved bone mass in the offspring with a more pronounced effect in females than males at adulthood

    DOI: 10.1016/j.jnutbio.2022.109245
    Sample: plasma
  4. Stem Cell Research & Therapy (2021) IF: 6.832
    Andrographolide promotes proliferative and osteogenic potentials of human placenta-derived mesenchymal stem cells through the activation of Wnt/β-catenin signaling

    DOI: 10.1186/s13287-021-02312-x

    PMID: 33853681

    		 Sample: Cell sample
  5. Materials Science & Engineering C-Materials for Biological Applications (2018) IF: 5.08
    Biosilica incorporated 3D porous scaffolds for bone tissue engineering applications

    DOI: 10.1016/j.msec.2018.05.040
    Sample: Cell culture supernatant
  6. CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
    Rutin-Zn(II) complex promotes bone formation - A concise assessment in human dental pulp stem cells and zebrafish

    DOI: 10.1016/j.cbi.2021.109674

    PMID: 34562440

    		 Sample: hDPSC
  7. RARE METALS (2021) IF: 4.003
    Degradation and biological performance of porous osteomimetic biphasic calcium phosphate in vitro and in vivo

    DOI: 10.1007/s12598-021-01814-0
    Sample: MG63 cell
  8. Biomolecules (2020) IF: 4.082
    Salivary Osteocalcin as Potential Diagnostic Marker of Periodontal Bone Destruction among Smokers

    DOI: 10.3390/biom10030380
    Sample: saliva
  9. BONE (2019) IF: 4.36
    Daily oligofructose-enriched inulin intake impacts bone turnover markers but not the cytokine profile in pediatric patients with celiac disease on a gluten-free diet: Results of a randomised, placebo-controlled pilot study

    DOI: 10.1016/j.bone.2019.03.001
    Sample: Plasma
  10. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2021) IF: 4.384
    Bioactive Zinc(II) complex incorporated PCL/gelatin electrospun nanofiber enhanced bone tissue regeneration

    DOI: 10.1016/j.ejps.2021.105768

    PMID: 33607242

    		 Sample: Cell culture supernatant(human MG-63 cells)
  11. JOURNAL OF CELLULAR BIOCHEMISTRY (2018) IF: 4.429
    In vivo and in vitro effects of PTH1‐34 on osteogenic and adipogenic differentiation of human bone marrow‐derived mesenchymal stem cells through regulating microRNA‐155

    DOI: 10.1002/jcb.26478
    Sample: Cell lysate
  12. BONE (2018) IF: 4.455
    Bone and plasma citrate is reduced in osteoporosis

    DOI: 10.1016/j.bone.2018.06.014
    Sample: serum
  13. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019) IF: 4.784
    The effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffolds

    DOI: 10.1016/j.ijbiomac.2019.10.213
    Sample: MG-63 cells
  14. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019) IF: 4.784
    Chitosan-hybrid poss nanocomposites for bone regeneration: The effect of poss nanocage on surface, morphology, structure and in vitro bioactivity

    DOI: 10.1016/j.ijbiomac.2019.10.006
    Sample: hFob cells,MG-63 cells,Saos-2 cells
  15. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019) IF: 4.784
    Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering

    DOI: 10.1016/j.ijbiomac.2019.02.067

    PMID: 30797008

    		 Sample: Cell culture medium
  16. Materials Science & Engineering C-Materials for Biological Applications (2019) IF: 4.959
    Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration

    DOI: 10.1016/j.msec.2019.110195

    PMID: 31761207

    		 Sample: Cell culture supernatant
  17. Materials Science & Engineering C-Materials for Biological Applications (2019) IF: 4.959
    Bioactive diatomite and POSS silica cage reinforced chitosan/Na-carboxymethyl cellulose polyelectrolyte scaffolds for hard tissue regeneration

    DOI: 10.1016/j.msec.2019.02.104

    PMID: 30948053

    		 Sample: Cell culture supernatant
  18. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY (2022) IF: 3.078
    Effects of 8-week increment aerobic exercise program on bone metabolism and body composition in young non-athletes

    DOI: 10.1007/s00421-022-04900-y

    PMID: 35141785

    		 Sample: serum
  19. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Chitosan based thermoresponsive hydrogel containing graphene oxide for bone tissue repair

    DOI: 10.1016/j.biopha.2018.08.072
    Sample: MG-63 cells
  20. OSTEOPOROSIS INTERNATIONAL (2017) IF: 3.591
    Vitamin D supplementation and bone turnover in advanced heart failure: the EVITA trial

    DOI: 10.1007/s00198-017-4312-9

    PMID: 29260292

    		 Sample: Plasma
  21. LIFE SCIENCES (2020) IF: 3.647
    Kaempferol-zinc(II) complex synthesis and evaluation of bone formation using zebrafish model

    DOI: 10.1016/j.lfs.2020.117993
    Sample: Cell culture medium
  22. LIFE SCIENCES (2020) IF: 3.647
    Assessment of human ovarian follicular fluid derived mesenchymal stem cells in chitosan/PCL/Zn scaffold for bone tissue regeneration

    DOI: 10.1016/j.lfs.2020.118502

    PMID: 33031825

    		 Sample: Cell sample
  23. Biomedical Materials (2021) IF: 3.715
    Bioactive snail mucus-slime extract loaded chitosan scaffolds for hard tissue regeneration: the effect of mucoadhesive and antibacterial extracts on physical characteristics and bioactivity of chitosan matrix

    DOI: 10.1088/1748-605X/ac2352
    Sample: Saos-2 cell
  24. PEPTIDES (2023) IF: 3.867
    Glucagon-like peptide-1 receptor promotes osteoblast differentiation of dental pulp stem cells and bone formation in a zebrafish scale regeneration model

    DOI: 10.1016/j.peptides.2023.170974
    Sample: Human dental pulp stem cells (hDPSCs)
  25. JOURNAL OF NEPHROLOGY (2021) IF: 3.902
    Body composition, adipokines, FGF23-Klotho and bone in kidney transplantation: Is there a link?

    DOI: 10.1007/s40620-021-00972-9

    PMID: 33560479

    		 Sample: Serum
  26. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2022) IF: 3.981
    Nano-encapsulation of melatonin into polydiacetylene-phospholipid assembly for sustained-release and enhanced bone formation in zebrafish

    DOI: 10.1016/j.jddst.2022.103415
    Sample: mMSCs
  27. JOURNAL OF BIOMATERIALS APPLICATIONS (2018) IF: 2.082
    Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite:

    DOI: 10.1177/0885328218811040
    Sample: Cell culture medium
  28. Applied Sciences-Basel (2021) IF: 2.679
    Biochemical Mapping of the Inflamed Human Dental Pulp

    DOI: 10.3390/app112110395
    Sample: dental pulp lysates
  29. Applied Sciences-Basel (2022) IF: 2.679
    Assessment of Mineralization, Oxidative Stress, and Inflammation Mechanisms in the Pulp of Primary Teeth

    DOI: 10.3390/app12031554
    Sample: Dental pulp tissue
  30. PROCESS BIOCHEMISTRY (2019) IF: 2.883
    Zinc chelated morin promotes osteoblast differentiation over its uncomplexed counterpart

    DOI: 10.1016/j.procbio.2019.04.008
    Sample: MG-63 cells
  31. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2015) IF: 1.006
    Neuropeptide Y, substance P, and human bone morphogenetic protein 2 stimulate human osteoblast osteogenic activity by enhancing gap junction intercellular communication

    DOI: 10.1590/1414-431x20144226
    Sample: Cell culture supernatant
  32. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2016) IF: 1.146
    Effect of magnesium ion on human osteoblast activity

    DOI: 10.1590/1414-431X20165257
    Sample: Cell culture supernatant
  33. Hematology (2019) IF: 1.318
    Disordered bone metabolism in hereditary spherocytosis patients

    DOI: 10.1080/16078454.2019.1565150

    PMID: 31793406

    		 Sample: Serum
  34. Endokrynologia Polska (2021) IF: 1.582
    New insights into the metabolic-bone crosstalk in active acromegaly

    DOI: 10.5603/EP.a2021.0028
    Sample: serum
  35. Biocatalysis and Agricultural Biotechnology (2020)
    Prophylactic supplementation of sinapic acid ameliorates zoledronic acid induced changes in osteoblast survival and differentiation

    DOI: 10.1016/j.bcab.2020.101700
    Sample: MG63 cell
  36. Journal of Dental Sciences (2019) IF: 0.798
    Diagnostic accuracy of salivary biomarkers of bone turnover in identifying patients with periodontitis in a Saudi Arabian population

    DOI: 10.1016/j.jds.2019.03.002

    PMID: 31534637

    		 Sample: Saliva
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