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Human PTX 3/TSG-14(Pentraxin 3) ELISA Kit

Citations(5)Uniprot : P26022

Cat.No.:E-EL-H6081
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	6.86-5000 pg/mL
Sensitivity	4.12 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human PTX3/TSG-14 in samples. No significant cross-reactivity or interference between Human PTX3/TSG-14 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human PTX3/TSG-14 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTX3/TSG-14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTX3/TSG-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTX3/TSG-14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTX3/TSG-14. You can calculate the concentration of Human PTX3/TSG-14 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PTX3/TSG-14 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PTX3/TSG-14 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	230.55	565.35	2010.46	209.00	617.46	1987.07
Standard deviation	13.63	24.99	96.70	13.63	29.64	104.52
CV (%)	5.91	4.42	4.81	6.52	4.80	5.26

Recovery

The recovery of Human PTX3/TSG-14 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-102	93
EDTA plasma (n=8)	90-104	96
Cell culture media (n=8)	86-102	93

Linearity

Samples were spiked with high concentrations of Human PTX3/TSG-14 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P26022
Synonyms	PTX3, TNFAIP5, TSG-14,Pentraxin 3, long,TNF-inducible gene 14 protein
Research Area	Cardiovascular, Immunology, Cancer, Cardiovascular

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cell Death & Disease (2022) IF: 8.4689
Pentraxin 3 regulated by miR-224-5p modulates macrophage reprogramming and exacerbates osteoarthritis associated synovitis by targeting CD32

DOI: 10.1038/s41419-022-04962-y

PMID: 35739102
Sample: serum,synovial fluid
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2022) IF: 4.925
Potential Therapeutic Activity of Berberine in Thyroid-Associated Ophthalmopathy: Inhibitory Effects on Tissue Remodeling in Orbital Fibroblasts

DOI: 10.1167/iovs.63.10.6

PMID: 36094643
Sample: Orbital fibroblasts (OFs)
JOURNAL OF MEDICAL VIROLOGY (2021) IF: 2.327
Evaluation of the relationship between pentraxin 3 (PTX3) rs2305619 (281A/G) and rs1840680 (1449A/G) polymorphisms and the clinical course of COVID-19

DOI: 10.1002/jmv.27238

PMID: 34314051
Sample: blood
PEDIATRICS INTERNATIONAL (2020) IF: 1.139
Determination of pentraxin 3 levels in diagnosis of appendicitis in children

DOI: 10.1111/ped.14131

Sample: serum
JOURNAL OF CLINICAL LABORATORY ANALYSIS (2018) IF: 1.303
Serum pentraxin‐3 levels and flow‐mediated dilation in dipper and non‐dipper hypertension

DOI: 10.1002/jcla.22718

Sample: Serum

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