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Cat.No.:E-EL-M2411
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Format | 24T, 48T, 96T, 96T*5, 96T*10 |
Assay time | 3.5h |
Sample type | serum, plasma and other biological fluids |
Sample volume | 100μL |
Sample size | Run up to 46 samples in duplicate and 30 samples in triplicate |
Storage | 2-8℃ |
Interpretation | Detection range: 0.03-20 ng/mL Sensitivity: 0.02 ng/mL |
Application | Quantitative determination of Human concentrations in human serum, plasma and other biological fluids |
Reproducibility | Both intra-CV and inter-CV are < 10% |
Synonyms | B2MG, B2M |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
DOI: 10.1155/2016/1786789 |
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Sample: Urinary |
Sample: serum |