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Mouse D2D(D-Dimer) ELISA Kit

Cat.No.:E-EL-M0400
Reactivity: Mouse

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96T*10

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Properties

Format	24T, 48T, 96T, 96T5, 96T10
Assay time	2.0h
Sample type	plasma
Sample volume	50μL
Sample size	Run up to 46 samples in duplicate and 30 samples in triplicate
Storage	2-8℃
Interpretation	Detection range: 78.13-5000 ng/mL Sensitivity: 46.88 ng/mL
Application	Quantitative determination of Mouse concentrations in mouse plasma
Reproducibility	Both intra-CV and inter-CV are < 10%

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse D2D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse D2D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse D2D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse D2D. You can calculate the concentration of Mouse D2D in the samples by comparing the OD of the samples to the standard curve.

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

Nature Chemical Biology (2022) IF: 16.174
Programmable DARPin-based receptors for the detection of thrombotic markers

DOI: 10.1038/s41589-022-01095-3

PMID: 35941237
Sample: plasma
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020) IF: 9.4120
Consumptive coagulopathy of severe yellow fever occurs independently of hepatocellular tropism and massive hepatic injury

DOI: 10.1073/pnas.2014096117

PMID: 33268494
Sample: plasma
BIOMATERIALS (2016) IF: 8.387
Fibrin degradation by rtPA enhances the delivery of nanotherapeutics to A549 tumors in nude mice

DOI: 10.1016/j.biomaterials.2016.04.015

Sample: Tissue homogenate
Blood Advances (2022) IF: 6.799
CD44-fibrinogen binding promotes bleeding in acute promyelocytic leukemia by in situ fibrin(ogen) deposition

DOI: 10.1182/bloodadvances.2022006980

PMID: 35511736
Sample: blood
Frontiers in Immunology (2019) IF: 4.716
Oral Route Driven Acute Trypanosoma cruzi Infection Unravels an IL-6 Dependent Hemostatic Derangement

DOI: 10.3389/fimmu.2019.01073

Sample: serum
BIOCHEMICAL PHARMACOLOGY (2019) IF: 4.825
Scavenger receptor A1 attenuates aortic dissection via promoting efferocytosis in macrophages

DOI: 10.1016/j.bcp.2019.07.027

PMID: 31381873
Sample: Whole Blood
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023) IF: 3.322
Administration of spermidine attenuates concanavalin A-induced liver injury

DOI: 10.1016/j.bbrc.2023.01.072

Sample: serum
BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2019) IF: 1.917
Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice

DOI: 10.3967/bes2018.101

Sample: Plasma,Tissue homogenate
Intensive Care Medicine Experimental (2022)
The effect of shock duration on trauma-induced coagulopathy in a murine model

DOI: 10.1186/s40635-021-00428-1

Sample: blood

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