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Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit

Citations(32)

  • Cat.No.:E-EL-R1430

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes Ang-Ⅱ in samples. No significant cross-reactivity or interference between Ang-Ⅱ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Ang-Ⅱ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅱ. During the reaction, Ang-Ⅱ in the sample or standard competes with a fixed amount of Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Ang-Ⅱ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Ang-Ⅱ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 46.80 133.70 360.10 46.10 133.40 382.00
Standard deviation 3.00 7.60 13.00 3.20 7.10 15.70
CV (%) 6.41 5.68 3.61 6.94 5.32 4.11

Recovery

The recovery of Ang-Ⅱ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 94
EDTA plasma(n=8) 90-104 96
Cell culture media(n=8) 84-96 91

Linearity

Samples were spiked with high concentrations of Rat Ang-Ⅱ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsAng-II, Angiotensin-2
Research AreaCancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
elisa assay procedure 2

2. Aspirate and wash the plate for 3 times
elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 5

5. Add 50μL Stop Solution
elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    Controlled Hemorrhage Sensitizes Angiotensin II-Elicited Hypertension through Activation of the Brain Renin-Angiotensin System Independently of Endoplasmic Reticulum Stress

    DOI: 10.1155/2022/6371048

    PMID: 35069977

    		 Sample: plasma
  2. LIFE SCIENCES (2021) IF: 5.037
    CIHH protects the heart against left ventricular remodelling and myocardial fibrosis by balancing the renin-angiotensin system in SHR

    DOI: 10.1016/j.lfs.2021.119540

    PMID: 33930369

    		 Sample: serum,heart
  3. Frontiers in Medicine (2022) IF: 5.058
    Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.

    DOI: 10.3389/fmed.2022.953749
    Sample: plasma,renal tissue
  4. CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
    Targeting inflammation and redox aberrations by perindopril attenuates methotrexate-induced intestinal injury in rats: Role of TLR4/NF-κB and c-Fos/c-Jun pro-inflammatory pathways and PPAR-γ/SIRT1 cytoprotective signals

    DOI: 10.1016/j.cbi.2021.109732

    PMID: 34737150

    		 Sample: intestinal tissue
  5. Pharmaceuticals (2022) IF: 5.215
    Azithromycin Mitigates Cisplatin-Induced Lung Oxidative Stress, Inflammation and Necroptosis by Upregulating SIRT1, PPARγ, and Nrf2/HO-1 Signaling

    DOI: 10.3390/ph16010052
    Sample: lungs
  6. JOURNAL OF HYPERTENSION (2017) IF: 4.085
    Adjunctive therapy with statins reduces residual albuminuria/proteinuria and provides further renoprotection by downregulating the angiotensin II–AT1 pathway in hypertensive nephropathy

    DOI: 10.1097/HJH.0000000000001325
    Sample: kidney tissue
  7. Frontiers in Pharmacology (2020) IF: 4.225
    Perindopril Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy

    DOI: 10.3389/fphar.2020.00094

    PMID: 32153406

    		 Sample: Serum
  8. ENVIRONMENTAL POLLUTION (2018) IF: 4.358
    COPD rat model is more susceptible to cold stress and PM2.5 exposure and the underlying mechanism

    DOI: 10.1016/j.envpol.2018.05.034
    Sample: Tissue homogenate
  9. Frontiers in Physiology (2021) IF: 4.566
    Chronic Intermittent Hypobaric Hypoxia Decreases High Blood Pressure by Stabilizing the Vascular Renin-Angiotensin System in Spontaneously Hypertensive Rats

    DOI: 10.3389/fphys.2021.639454
    Sample: serum
  10. EXPERIMENTAL BIOLOGY AND MEDICINE (2019) IF: 3.005
    Calcimimetic R568 improved cardiac remodeling by classic and novel renin-angiotensin system in spontaneously hypertensive rats:

    DOI: 10.1177/1535370219854325
    Sample: plasm
  11. Journal of Functional Foods (2019) IF: 3.197
    The tannins from Punica granatum L, natural regulator of TGF-β1/Smad signaling activity improves nephrectomy and adriamycin induced focal segmental glomerulosclerosis in vivo

    DOI: 10.1016/j.jff.2019.04.021
    Sample: serum
  12. BIOCHIMIE (2019) IF: 3.362
    TLR2 and TLR4 mediate an activation of adipose tissue renin-angiotensin system induced by uric acid

    DOI: 10.1016/j.biochi.2019.04.013
    Sample: serum,adipocytes
  13. TOXICOLOGY AND APPLIED PHARMACOLOGY (2018) IF: 3.616
    Metformin attenuates cardiovascular and renal injury in uninephrectomized rats on DOCA-salt: Involvement of AMPK and miRNAs in cardioprotection

    DOI: 10.1016/j.taap.2018.10.004
    Sample: Plasma
  14. LIFE SCIENCES (2020) IF: 3.647
    Taxifolin improves disorders of glucose metabolism and water-salt metabolism in kidney via PI3K/AKT signaling pathway in metabolic syndrome rats

    DOI: 10.1016/j.lfs.2020.118713

    PMID: 33157091

    		 Sample: Serum,Urine
  15. Journal of Translational Medicine (2016) IF: 3.694
    Modulation of the sympathetic nervous system by renal denervation prevents reduction of aortic distensibility in atherosclerosis prone ApoE-deficient rats

    DOI: 10.1186/s12967-016-0914-9
    Sample: Tissue homogenate
  16. BIOLOGICAL TRACE ELEMENT RESEARCH (2021) IF: 3.738
    Effect of Telmisartan on Arsenic-Induced (Sub-chronic) Perturbations in Redox Homeostasis, Pro-inflammatory Cascade and Aortic Dysfunction in Wistar Rats

    DOI: 10.1007/s12011-021-02804-0

    PMID: 34339004

    		 Sample: plasma
  17. International Journal of Endocrinology (2019) IF: 2.287
    Aliskiren, Fosinopril, and Their Outcome on Renin-Angiotensin-Aldosterone System (RAAS) in Rats with Thyroid Dysfunction

    DOI: 10.1155/2019/5960563
    Sample: serum
  18. JOURNAL OF PHARMACY AND PHARMACOLOGY (2019) IF: 2.39
    The effect of eplerenone on the renin‐angiotensin‐aldosterone system of rats with thyroid dysfunction

    DOI: 10.1111/jphp.13168
    Sample: Serum
  19. Experimental and Therapeutic Medicine (2018) IF: 2.447
    Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin‑angiotensin system activity

    DOI: 10.3892/etm.2018.6734
    Sample: Plasma,Tissue homogenate
  20. Experimental and Therapeutic Medicine (2020) IF: 2.447
    Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system

    DOI: 10.3892/etm.2020.8759
    Sample: Cell lysate
  21. BIOMETALS (2020) IF: 2.479
    Cardio-protective effects of a dioxidovanadium(V) complex in male sprague–dawley rats with streptozotocin-induced diabetes

    DOI: 10.1007/s10534-020-00270-0
    Sample: Plasma
  22. BMC Complementary and Alternative Medicine (2019) IF: 2.479
    Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats

    DOI: 10.1186/s12906-019-2463-2

    PMID: 30819180

    		 Sample: Serum
  23. EUROPEAN JOURNAL OF PHARMACOLOGY (2016) IF: 2.73
    The protective role of neuregulin-1: A potential therapy for sepsis-induced cardiomyopathy

    DOI: 10.1016/j.ejphar.2016.06.042
    Sample: Serum
  24. FUNDAMENTAL & CLINICAL PHARMACOLOGY (2021) IF: 2.748
    Angiotensin aldosterone inhibitors improve survival and ameliorate kidney injury induced by sepsis through suppression of inflammation and apoptosis

    DOI: 10.1111/fcp.12718

    PMID: 34309069

    		 Sample: serum
  25. Pharmacological Reports (2019) IF: 2.761
    Simultaneous inhibition of neprilysin and activation of ACE2 prevented diabetic cardiomyopathy

    DOI: 10.1016/j.pharep.2019.05.008
    Sample: heart tissue，plasma
  26. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2018) IF: 2.776
    The probable roles of valsartan in alleviating chronic obstructive pulmonary disease following co-exposure to cold stress and fine particulate matter

    DOI: 10.1016/j.etap.2018.05.002
    Sample: Tissue homogenate
  27. PLoS One (2017) IF: 2.806
    Dysregulation of microRNAs and renin-angiotensin system in high salt diet-induced cardiac dysfunction in uninephrectomized rats

    DOI: 10.1371/journal.pone.0180490
    Sample: Plasma
  28. MOLECULES (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

    DOI: 10.3390/molecules22111956
    Sample: Cell culture supernatant
  29. Reviews in Cardiovascular Medicine (2021) IF: 2.93
    Histone deacetylase inhibitor, mocetinostat, regulates cardiac remodelling and renin-angiotensin system activity in rats with transverse aortic constriction-induced pressure overload cardiac hypertrophy.

    DOI: 10.31083/j.rcm2203113
    Sample: serum
  30. CLINICAL AND EXPERIMENTAL HYPERTENSION (2019) IF: 1.522
    Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats

    DOI: 10.1080/10641963.2018.1506467

    PMID: 30648895

    		 Sample: Serum
  31. BIOLOGICAL & PHARMACEUTICAL BULLETIN (2018) IF: 1.694
    Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

    DOI: 10.1248/bpb.b17-00930

    PMID: 30068867

    		 Sample: Plasma
  32. EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2019) IF: 1.927
    Hyperuricemia Inhibition Protects SD Rats Against Fructose-Induced Obesity Hypertension Via Modulation of Inflammation and Renin-Angiotensin System in Adipose Tissue

    DOI: 10.1055/a-1023-6710

    PMID: 31683330

    		 Sample: Serum
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