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Rat Cys-C(Cystatin C) ELISA Kit

Cat.No.:E-EL-R0304
Reactivity: Rat

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat Cys-C in samples. No significant cross-reactivity or interference between Rat Cys-C and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat Cys-C concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Cys-C. You can calculate the concentration of Rat Cys-C in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat Cys-C were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat Cys-C were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.10	4.00	0.50	1.10	4.20
Standard deviation	0.03	0.05	0.20	0.03	0.06	0.15
CV (%)	6.00	4.55	5.00	6.00	5.45	3.57

Recovery

The recovery of Rat Cys-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-104	96
EDTA plasma (n=8)	86-99	91
Cell culture media (n=8)	93-107	101

Linearity

Samples were spiked with high concentrations of Rat Cys-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CST3, Cystatin 3, ARMD11, Post-Gamma-Globulin
Research Area	Cancer, Cell Biology, Cardiovascular, Stem cells, Tags and Cell Markers

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Computational and Structural Biotechnology Journal (2020) IF: 6.018
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

DOI: 10.1016/j.csbj.2020.11.009

PMID: 33304464
Sample: blood
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2021) IF: 6.514
Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms

DOI: 10.1016/j.ejmech.2021.113388

PMID: 33784603
Sample: serum
LIFE SCIENCES (2022) IF: 5.037
Angiotensin 1–7 mitigates rhabdomyolysis induced renal injury in rats via modulation of TLR-4/NF-kB/iNOS and Nrf-2/heme‑oxygenase-1 signaling pathways

DOI: 10.1016/j.lfs.2022.120678

Sample: serum
Cell Death Discovery (2022) IF: 5.241
Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats

DOI: 10.1038/s41420-022-00822-y

PMID: 35022384
Sample: serum
Drug Design Development and Therapy (2022) IF: 4.319
Alpha-Chymotrypsin Protects Against Acute Lung, Kidney, and Liver Injuries and Increases Survival in CLP-Induced Sepsis in Rats Through Inhibition of TLR4/NF-κB Pathway

DOI: 10.2147/DDDT.S370460

Sample: serum
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
Nephroprotective mechanisms of Ambrette (Abelmoschus moschatus Medik.) leaf extracts in adriamycin mediated acute kidney injury model of Wistar rats

DOI: 10.1016/j.jep.2022.115221

PMID: 35339624
Sample: Kidney
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2016) IF: 4.652
Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy

DOI: 10.1159/000452561

Sample: Plasma,Tissue homogenate
Biomedicines (2022) IF: 4.757
Effect of Celecoxib and Infliximab against Multiple Organ Damage Induced by Sepsis in Rats: A Comparative Study

DOI: 10.3390/biomedicines10071613

PMID: 35884918
Sample: Serum
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2022) IF: 4.86
Protective effect of empagliflozin on gentamicin-induced acute renal injury via regulation of SIRT1/NF-κB signaling pathway

DOI: 10.1016/j.etap.2022.103907

PMID: 35697188
Sample: serum
Toxicology Research (2022) IF: 3.524
Cerium oxide nanoparticles attenuate the renal injury induced by cadmium chloride via improvement of the NBN and Nrf2 gene expressions in rats

DOI: 10.1093/toxres/tfac009

PMID: 35510236
Sample: kidney
JOURNAL OF PHARMACY AND PHARMACOLOGY (2021) IF: 3.765
Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress

DOI: 10.1093/jpp/rgab091

Sample: serum
FUNDAMENTAL & CLINICAL PHARMACOLOGY (2021) IF: 2.748
Angiotensin aldosterone inhibitors improve survival and ameliorate kidney injury induced by sepsis through suppression of inflammation and apoptosis

DOI: 10.1111/fcp.12718

PMID: 34309069
Sample: serum
Experimental and Therapeutic Medicine (2017) IF: 1.261
Mechanism of myocardial ischemia/reperfusion‑induced acute kidney injury through DJ‑1/Nrf2 pathway in diabetic rats

DOI: 10.3892/etm.2017.5095

Sample: Serum
BIOLOGICAL & PHARMACEUTICAL BULLETIN (2018) IF: 1.694
Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

DOI: 10.1248/bpb.b17-00930

PMID: 30068867
Sample: Plasma
RENAL FAILURE (2015) IF: 0.944
The role of DJ-1/Nrf2 pathway in the pathogenesis of diabetic nephropathy in rats

DOI: 10.3109/0886022X.2015.1120119

Sample: Serum
Acta Cirurgica Brasileira (2020) IF: 0.974
Effects of ATRA on diabetic rats with renal ischemia-reperfusion injury

DOI: 10.1590/s0102-865020200010000006

PMID: 32236320
Sample: Serum

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