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Rat EGF(Epidermal Growth Factor) ELISA Kit

Name: Rat EGF(Epidermal Growth Factor) ELISA Kit
Brand: Elabscience
SKU: E-EL-R0369
Price: 495 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(8)

Cat.No.:E-EL-R0369
Reactivity: Rat

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat EGF in samples. No significant cross-reactivity or interference between Rat EGF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat EGF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat EGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat EGF. You can calculate the concentration of Rat EGF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat EGF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat EGF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	55.10	94.40	445.80	57.60	94.60	475.90
Standard deviation	3.40	5.30	15.20	3.20	5.60	19.00
CV (%)	6.17	5.61	3.41	5.56	5.92	3.99

Recovery

The recovery of Rat EGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-99	92
EDTA plasma (n=8)	93-107	99
Cell culture media (n=8)	93-107	100

Linearity

Samples were spiked with high concentrations of Rat EGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	URG, HOMG4, Beta-Urogastrone
Research Area	Cancer, Cell Biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF CONTROLLED RELEASE (2020) IF: 7.727
Photo-responsive supramolecular hyaluronic acid hydrogels for accelerated wound healing

DOI: 10.1016/j.jconrel.2020.04.014

PMID: 32283209
Sample: Tissue homogenate
PHYTOMEDICINE (2021) IF: 5.34
Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

DOI: 10.1016/j.phymed.2021.153875

PMID: 34911003
Sample: serum
Scientific Reports (2021) IF: 4.38
A nano phototheranostic approach of toluidine blue conjugated gold silver core shells mediated photodynamic therapy to treat diabetic foot ulcer

DOI: 10.1038/s41598-021-04008-x

PMID: 34961769
Sample: serum
PLANTA MEDICA (2022) IF: 3.007
Curative effect and mechanisms of Radix Arnebiae oil on burn wound healing in rats

DOI: 10.1055/a-1997-5566

Sample: burn wound tissue
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2022) IF: 2.723
Impact of repeated intravenous infusions of umbilical cord–derived versus bone marrow–derived mesenchymal stem cells on angiogenesis in a pregnant experimentally induced deep venous thrombosis rat model

DOI: 10.1007/s11626-022-00733-3

Sample: serum
Annals of Surgical Treatment and Research (2019) IF: 1.181
Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

DOI: 10.4174/astr.2019.97.6.271

PMID: 31824881
Sample: Serum,Tissue homogenate
Annals of Transplantation (2021) IF: 1.53
Amniotic Fluid Transplantation Alleviates Hematopoietic Deficits in Experimental Rat Aplastic Anemia

DOI: 10.12659/AOT.928047

PMID: 33414361
Sample: The amniotic fluid samples
Asian Journal of Surgery (2019) IF: 1.56
Potent therapeutic effects of ruscogenin on gastric ulcer established by acetic acid

DOI: 10.1016/j.asjsur.2019.07.001

PMID: 31345657
Sample: Tissue homogenate

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Verified Customer

h*********aSubmitted [ Jun 17 2019 ]

Species:Human
Sample:Rat urine
Sample source:Rat urine
Description:The ELISA Kit of Rat EGF I bought to detect Rat urine . The result is helpful to my subject . Rat EGF concentration of urine is high . it’s more sensitive than serum value . In the process , CSD help me a lot . thy are so patient . although I made a mistake at first . they found it and correct it .I got the satisfying answer in final . I am very grateful .

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