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Rat LEP(Leptin) ELISA Kit

Citations(41)Uniprot : P50596

  • Cat.No.:E-EL-R0582

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat LEP in samples. No significant cross-reactivity or interference between Rat LEP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat LEP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LEP. You can calculate the concentration of Rat LEP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat LEP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat LEP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.50 4.40 0.50 1.50 4.80
Standard deviation 0.03 0.08 0.17 0.03 0.08 0.23
CV (%) 6.00 5.33 3.86 6.00 5.33 4.79

Recovery

The recovery of Rat LEP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-107 101
EDTA plasma (n=8) 85-98 91
Cell culture media (n=8) 95-109 102

Linearity

Samples were spiked with high concentrations of Rat LEP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P50596
SynonymsLEPD, OB, OBS, Obesity Homolog
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Antioxidants (2021) IF: 6.313
    Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model

    DOI: 10.3390/antiox10020251

    PMID: 33562069

    		 Sample: Serum
  2. MOLECULAR MEDICINE (2022) IF: 6.376
    Hypolipidemic effect of ethanol extract from Chimonanthus nitens Oliv. leaves in hyperlipidemia rats via activation of the leptin/JAK2/STAT3 pathway

    DOI: 10.1186/s10020-022-00589-z
    Sample: serum
  3. Frontiers in Cell and Developmental Biology (2022) IF: 6.684
    Maternal Diabetes and Postnatal High-Fat Diet on Pregnant Offspring.

    DOI: 10.3389/fcell.2022.818621
    Sample: maternal,placental,,fetal
  4. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2022) IF: 5.011
    Acetate: A therapeutic candidate against renal disorder in a rat model of polycystic ovarian syndrome

    DOI: 10.1016/j.jsbmb.2022.106179

    PMID: 36150640

    		 Sample: plasma
  5. Food & Function (2022) IF: 5.396
    L-Methionine supplementation attenuates high-fat fructose diet-induced non-alcoholic steatohepatitis by modulating lipid metabolism, fibrosis and inflammation in rats

    DOI: 10.1039/D1FO03403K
    Sample: plasma
  6. Frontiers in Pharmacology (2021) IF: 5.811
    Hydroethanolic Extract of A. officinarum Hance Ameliorates Hypertension and Causes Diuresis in Obesogenic Feed-Fed Rat Model

    DOI: 10.3389/fphar.2021.670433
    Sample: serum
  7. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2021) IF: 4.31
    Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet

    DOI: 10.1152/ajpendo.00093.2021

    PMID: 34229480

    		 Sample: serum,testis
  8. Scientific Reports (2022) IF: 4.38
    Gold nanoclusters eliminate obesity induced by antipsychotics

    DOI: 10.1038/s41598-022-09541-x
    Sample: plasma
  9. Scientific Reports (2021) IF: 4.38
    Acetate rescues defective brain-adipose metabolic network in obese Wistar rats by modulation of peroxisome proliferator-activated receptor-γ

    DOI: 10.1038/s41598-021-98605-5

    PMID: 34556775

    		 Sample: plasma,brain tissue,adipose tissue
  10. SAUDI PHARMACEUTICAL JOURNAL (2023) IF: 4.562
    6-gingerol ameliorates weight gain and insulin resistance in metabolic syndrome rats by regulating adipocytokines

    DOI: 10.1016/j.jsps.2023.01.003
    Sample: serum,adipose tissues
  11. PSYCHONEUROENDOCRINOLOGY (2022) IF: 4.693
    Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats

    DOI: 10.1016/j.psyneuen.2022.105862

    PMID: 35835020

    		 Sample: Serum
  12. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2020) IF: 4.873
    Fish oil and corn oil induced differential effect on beiging of visceral and subcutaneous white adipose tissue in high-fat-diet-induced obesity

    DOI: 10.1016/j.jnutbio.2020.108458
    Sample: Serum
  13. NUTRITION (2022) IF: 4.893
    Acetate circumvents impaired metabolic switch in skeletal muscle of letrozole-induced PCOS rat model by suppression of PDK4/NLRP3

    DOI: 10.1016/j.nut.2022.111914
    Sample: plasma
  14. Drug Design Development and Therapy (2015) IF: 3.028
    Edible bird’s nest attenuates procoagulation effects of high-fat diet in rats

    DOI: 10.2147/DDDT.S87772
    Sample: Serum
  15. Food & Nutrition Research (2016) IF: 3.226
    Perinatal exposure to germinated brown rice and its gamma amino-butyric acid-rich extract prevents high fat diet-induced insulin resistance in first generation rat offspring

    DOI: 10.3402/fnr.v60.30209
    Sample: Serum
  16. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance

    DOI: 10.1016/j.etap.2020.103506

    PMID: 33002592

    		 Sample: Serum
  17. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    		 Sample: Serum
  18. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2015) IF: 3.412
    Protective effects of metformin on reproductive function in obese male rats induced by high-fat diet

    DOI: 10.1007/s10815-015-0506-2
    Sample: Serum
  19. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Curcumin nanoemulsion counteracts hepatic and cardiac complications associated with high-fat/high-fructose diet in rats

    DOI: 10.1111/jfbc.14442

    PMID: 36165438

    		 Sample: serum
  20. BMC Complementary and Alternative Medicine (2022) IF: 3.659
    Attenuation of carbohydrate metabolism and lipid profile by methanolic extract of Euphorbia helioscopia and improvement of beta cell function in a type 2 diabetic rat model

    DOI: 10.1186/s12906-022-03507-2

    PMID: 35078449

    		 Sample: serum
  21. JOURNAL OF PHARMACY AND PHARMACOLOGY (2021) IF: 3.765
    A comparative study between curcumin and curcumin nanoemulsion on high-fat, high-fructose diet-induced impaired spermatogenesis in rats

    DOI: 10.1093/jpp/rgab172

    PMID: 34928371

    		 Sample: serum
  22. PHYTOTHERAPY RESEARCH (2019) IF: 3.766
    Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity‐related endothelial dysfunction in rat fed a high‐fat diet

    DOI: 10.1002/ptr.6369
    Sample: blood
  23. NUTRITION RESEARCH (2022) IF: 3.876
    Pine nut oil supplementation alleviates the obesogenic effects in high-fat diet induced obese rats: A comparative study between epididymal and retroperitoneal adipose tissue.

    DOI: 10.1016/j.nutres.2022.07.003
    Sample: serum
  24. Scientific Reports (2020) IF: 3.998
    Effect of different levels of hydrolysable tannin intake on the reproductive hormones and serum biochemical indices in healthy female rats

    DOI: 10.1038/s41598-020-77672-0

    PMID: 33244120

    		 Sample: serum
  25. Journal of Developmental Origins of Health and Disease (2017) IF: 2.07
    Effect of neonatal orally administered S-allyl cysteine in high-fructose diet fed Wistar rats

    DOI: 10.1017/S2040174417000940
    Sample: Plasma
  26. Food & Nutrition Research (2015) IF: 2.162
    N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats

    DOI: 10.3402/fnr.v59.29046
    Sample: Serum
  27. Journal of Diabetes Research (2015) IF: 2.164
    Edible Bird’s Nest Prevents High Fat Diet-Induced Insulin Resistance in Rats

    DOI: 10.1155/2015/760535
    Sample: Serum
  28. Islets (2017) IF: 2.2
    The adverse effects of chronic low-dose exposure to nonylphenol on type 2 diabetes mellitus in high sucrose-high fat diet-treated rats

    DOI: 10.1080/19382014.2017.1404211
    Sample: Plasma
  29. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018) IF: 2.21
    Combined treatment with interleukin-1 and tumor necrosis factor-alpha antagonists improve type 2 diabetes in rats

    DOI: 10.1139/cjpp-2017-0769
    Sample: Serum
  30. BMC Complementary and Alternative Medicine (2017) IF: 2.288
    In utero exposure to germinated brown rice and its oryzanol-rich extract attenuated high fat diet-induced insulin resistance in F1 generation of rats

    DOI: 10.1186/s12906-017-1571-0
    Sample: Serum
  31. SOUTH AFRICAN JOURNAL OF BOTANY (2021) IF: 2.315
    Investigation of therapeutic potential of three endemic Cirsium species for global health problem obesity

    DOI: 10.1016/j.sajb.2021.05.004
    Sample: Serum
  32. Journal of Orthopaedic Surgery and Research (2021) IF: 2.359
    Treadmill running induces remodeling of the infrapatellar fat pad in an intensity-dependent manner

    DOI: 10.1186/s13018-021-02501-7

    PMID: 34074301

    		 Sample: serum
  33. Andrology (2020) IF: 2.86
    Orlistat attenuates obesity‐induced decline in steroidogenesis and spermatogenesis by up‐regulating steroidogenic genes

    DOI: 10.1111/andr.12824
    Sample: Tissue homogenate
  34. Molecular Medicine Reports (2016) IF: 2.952
    Curcumin ameliorates high‑fat diet‑induced spermatogenesis dysfunction

    DOI: 10.3892/mmr.2016.5712
    Sample: Serum
  35. Biomed Research International (2015) IF: 1.579
    N-Acetylneuraminic Acid Supplementation Prevents High Fat Diet-Induced Insulin Resistance in Rats through Transcriptional and Nontranscriptional Mechanisms

    DOI: 10.1155/2015/602313
    Sample: Serum
  36. BIOLOGIA (2022) IF: 1.653
    The effects of β-escin on inflammation, oxidative stress and Langerhans islet cells in high-fat diet and streptozotocin injection induced experimental type-2 diabetes model

    DOI: 10.1007/s11756-022-01266-6
    Sample: serum
  37. BIOTECHNIC & HISTOCHEMISTRY (2021) IF: 1.718
    Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones

    DOI: 10.1080/10520295.2021.1896780

    PMID: 33722110

    		 Sample: serum
  38. Heliyon (2021)
    Sildenafil ameliorates leptin resistance and normalizes lipid handling in the hypothalamic and adipose tissues of testosterone-exposed pregnant rats

    DOI: 10.1016/j.heliyon.2021.e07574
    Sample: plasma
  39. Middle East Fertility Society Journal (2016)
    Combined high dose vitamin C and E increases oxidative stress and visceral fat mass in rats treated by depot-medroxyprogesterone acetate

    DOI: 10.1016/j.mefs.2016.06.001
    Sample: serum
  40. Journal of Diabetes and Metabolic Disorders (2021)
    Acetate supplementation restores testicular function by modulating Nrf2/PPAR-γ in high fat diet-induced obesity in Wistar rats

    DOI: 10.1007/s40200-021-00924-x
    Sample: plasma
  41. Asian Pacific Journal of Tropical Medicine (2016) IF: 0.841
    Changes of adipocytokine expression after diabetic rats received sitagliptin and the molecular mechanism

    DOI: 10.1016/j.apjtm.2016.07.014
    Sample: Serum
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