Click here to view cell apoptosis assay products
×

Current Position:Home>>Kits>>ELISA Kits>> Rat PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Rat PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Citations(24)

  • Cat.No.:E-EL-R0792

  • Reactivity: Rat
Filter:

Manual MSDS

To Purchase E-EL-R0792

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

 Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 125.00-8000 pg/mL
Sensitivity 75.00 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Rat PTGS2/COX-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PTGS2/COX-2. You can calculate the concentration of Rat PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat PTGS2/COX-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat PTGS2/COX-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 403.31 795.28 3388.82 400.56 815.30 3160.09
Standard deviation 27.43 46.92 102.34 26.44 41.17 165.59
CV (%) 6.80 5.90 3.02 6.60 5.05 5.24

Recovery

The recovery of Rat PTGS2/COX-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-96 90
EDTA plasma (n=8) 90-103 97
Cell culture media (n=8) 91-103 97

Linearity

Samples were spiked with high concentrations of Rat PTGS2/COX-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCOX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Research AreaCancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Carveol Attenuates Seizure Severity and Neuroinflammation in Pentylenetetrazole-Kindled Epileptic Rats by Regulating the Nrf2 Signaling Pathway

    DOI: 10.1155/2021/9966663

    PMID: 34422216

    		 Sample: brain
  2. Frontiers in Nutrition (2022) IF: 6.576
    Comparative Analysis of the Bioactive Compounds in Chicken Cartilage: Protective Effects of Chondroitin Sulfate and Type II Collagen Peptides Against Osteoarthritis Involve Gut Microbiota

    DOI: 10.3389/fnut.2022.843360
    Sample: synovial fluid,serum
  3. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 6.953
    Metabolites of gut microbiota fermenting Poria cocos polysaccharide alleviates chronic nonbacterial prostatitis in rats

    DOI: 10.1016/j.ijbiomac.2022.04.029

    PMID: 35398386

    		 Sample: prostate
  4. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020) IF: 5.162
    Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management

    DOI: 10.1016/j.ijbiomac.2020.07.133

    PMID: 32693125

    		 Sample: Serum
  5. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 5.19
    Mechanistic insights on the possible protective role of polyphenols extracted from Tamarix aphylla aerial parts against sodium arsenite-induced hepatotoxicity in rats

    DOI: 10.1007/s11356-022-23324-x

    PMID: 36190635

    		 Sample: serum
  6. Frontiers in Endocrinology (2021) IF: 5.555
    The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats

    DOI: 10.3389/fendo.2021.652807
    Sample: plasma
  7. NEUROTOXICOLOGY (2022) IF: 4.398
    Carveol ameliorates mercury-induced oxidative stress, neuroinflammation, and neurodegeneration in a mouse brain

    DOI: 10.1016/j.neuro.2022.08.006

    PMID: 35963490

    		 Sample: brain
  8. REDOX REPORT (2022) IF: 4.412
    Suppression of glutathione system and upregulation of caspase 3-dependent apoptosis mediate rohypnol-induced gastric injury

    DOI: 10.1080/13510002.2022.2074128

    PMID: 35535549

    		 Sample: gastric tissue
  9. MOLECULES (2022) IF: 4.412
    Metabolomic Profiling Reveals Protective Effects and Mechanisms of Sea Buckthorn Sterol against Carbon Tetrachloride-Induced Acute Liver Injury in Rats

    DOI: 10.3390/molecules27072224

    PMID: 35408620

    		 Sample: Liver tissues
  10. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats

    DOI: 10.1016/j.ejphar.2021.174044

    PMID: 33745959

    		 Sample: Tissue homogenate(Joint Tissue)
  11. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats

    DOI: 10.1007/s10787-020-00785-5

    PMID: 33459879

    		 Sample: Tissue homogenate(hippocampus)
  12. Aging-US (2020) IF: 4.831
    Cyclooxygenase-2/sclerostin mediates TGF-β1-induced calcification in vascular smooth muscle cells and rats undergoing renal failure

    DOI: 10.18632/aging.103827

    PMID: 33159018

    		 Sample: Cell lysate
  13. LASERS IN SURGERY AND MEDICINE (2019) IF: 3.262
    Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

    DOI: 10.1002/lsm.23156
    Sample: Tissue homogenate
  14. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: serum
  15. LIFE SCIENCES (2020) IF: 3.647
    Beneficial effects of ferulic acid alone and in combination with insulin in streptozotocin induced diabetic neuropathy in Sprague Dawley rats

    DOI: 10.1016/j.lfs.2020.117856
    Sample: Tissue homogenate
  16. INFLAMMOPHARMACOLOGY (2019) IF: 3.838
    Zinc and iron complexes of oleanolic acid, (OA) attenuate allergic airway inflammation in rats

    DOI: 10.1007/s10787-019-00597-2
    Sample: serum
  17. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2017) IF: 2.042
    Protective effect of proanthocyanidins against doxorubicin‐induced nephrotoxicity in rats

    DOI: 10.1002/jbt.21965
    Sample: Tissue homogenate
  18. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2017) IF: 2.042
    Alpha lipoic acid prevents doxorubicin‐induced nephrotoxicity by mitigation of oxidative stress, inflammation, and apoptosis in rats

    DOI: 10.1002/jbt.21940
    Sample: Tissue homogenate
  19. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
    Protective effect of oleuropein on ketamine-induced cardiotoxicity in rats

    DOI: 10.1007/s00210-020-01870-w

    PMID: 32383030

    		 Sample: Serum
  20. Iranian Journal of Basic Medical Sciences (2020) IF: 2.146
    Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain

    DOI: 10.22038/ijbms.2020.45193.10527

    PMID: 33149866

    		 Sample: Tissue homogenate
  21. MOLECULES (2016) IF: 2.465
    Zerumbone, a Bioactive Sesquiterpene, Ameliorates Diabetes-Induced Retinal Microvascular Damage through Inhibition of Phospho-p38 Mitogen-Activated Protein Kinase and Nuclear Factor-κB Pathways

    DOI: 10.3390/molecules21121708
    Sample: Tissue homogenate
  22. MOLECULES (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

    DOI: 10.3390/molecules22111956
    Sample: Cell culture supernatant
  23. INFLAMMATION (2019) IF: 2.939
    Protective Effects of Tyrosol Against DSS-Induced Ulcerative Colitis in Rats

    DOI: 10.1007/s10753-019-01028-8
    Sample: colon tissue
  24. Acta Cirurgica Brasileira (2018) IF: 1.388
    Effect of ozone oxidative preconditioning on inflammation and oxidative stress injury in rat model of renal transplantation

    DOI: 10.1590/s0102-865020180030000006
    Sample: Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

About Us

Company Information

Distributors

Contact us

News

Return and Refund Policy

dhl Fedex
Terms and Conditions | Privacy Policy | Cookie Policy  ©2009-2024Elabscience Bionovation Inc. All Rights Reserved.
鄂ICP备18003807号-3
We use cookies to improve your browsing experience, serve personalized ads or content, and for analytics and metrics about our visitors both on this website and other media. By clicking "Accept All", you consent to our use of cookies. To find out more about the cookies we use, see our Cookie Policy
Accept All Reject All
Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?

*Captcha:
Click to load Captcha