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Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

Cat.No.:E-EL-R2603
Reactivity: Rat

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat VEGF-A in samples. No significant cross-reactivity or interference between Rat VEGF-A and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat VEGF-A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VEGF-A. You can calculate the concentration of Rat VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat VEGF-A were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	97.90	170.39	948.99	89.62	158.20	968.59
Standard deviation	5.02	9.25	46.60	5.65	7.26	37.97
CV (%)	5.13	5.43	4.91	6.30	4.59	3.92

Recovery

The recovery of Rat VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-101	93
EDTA plasma (n=8)	90-102	95
Cell culture media (n=8)	89-101	95

Linearity

Samples were spiked with high concentrations of Rat VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Bioactive Materials (2021) IF: 14.593
Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair

DOI: 10.1016/j.bioactmat.2021.03.020

PMID: 33842738
Sample: Cell culture supernatant
ADVANCED FUNCTIONAL MATERIALS (2018) IF: 13.325
MRI‐Visible siRNA Nanomedicine Directing Neuronal Differentiation of Neural Stem Cells in Stroke

DOI: 10.1002/adfm.201706769

Sample: neural stem cells (NSCs)
Advanced Fiber Materials (2022) IF: 12.958
Rock Climbing-Inspired Electrohydrodynamic Cryoprinting of Micropatterned Porous Fiber Scaffolds with Improved MSC Therapy for Wound Healing

DOI: 10.1007/s42765-022-00224-w
Advanced Healthcare Materials (2022) IF: 11.092
Spatiotemporal regulation of the bone immune microenvironment via dam-like biphasic bionic periosteum for bone regeneration

DOI: 10.1002/adhm.202201661

PMID: 36189833
Sample: BMSCs
BIOMATERIALS (2020) IF: 10.317
Exosome-mimetics as an engineered gene-activated matrix induces in-situ vascularized osteogenesis

DOI: 10.1016/j.biomaterials.2020.119985

Sample: Cell culture supernatant
Materials Today Advances (2023) IF: 9.9179
Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury

DOI: 10.1016/j.mtadv.2023.100349

Sample: bone marrow mesenchymal stem cells (BMSCs)
MATERIALS & DESIGN (2022) IF: 7.991
Mild photothermal effect of titania nanotubes array as a promising solution for peri-implantitis

DOI: 10.1016/j.matdes.2022.110641

Sample: EPCs
Biomedicines (2022) IF: 6.081
The Role of Neuregulin-1 in Steatotic and Non-Steatotic Liver Transplantation from Brain-Dead Donors

DOI: 10.3390/biomedicines10050978

PMID: 35625715
Sample: liver tissue
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208
SENP6-Mediated deSUMOylation of VEGFR2 Enhances Its Cell Membrane Transport in Angiogenesis

DOI: 10.3390/ijms24032544

Sample: retinal microvascular pericytes (RMPs)
Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
Potential Therapeutic Effects of Mi-Jian-Chang-Pu Decoction on Neurochemical and Metabolic Changes of Cerebral Ischemia-Reperfusion Injury in Rats

DOI: 10.1155/2022/7319563

PMID: 35578728
Sample: brain
PHYTOMEDICINE (2022) IF: 6.656
Integration of transdermal chemistry and network pharmacology to decipher the mechanism of ShexiangZhuifeng analgesic plaster to treat rheumatoid arthritis

DOI: 10.1016/j.phymed.2022.154507

PMID: 36334391
Sample: serum
LIFE SCIENCES (2022) IF: 6.78
Attenuated effect of zinc gluconate on oxidative stress, inflammation, and angiogenic imbalance in pre-eclampsia rats

DOI: 10.1016/j.lfs.2022.121055

PMID: 36228770
Sample: serum
Stem Cell Research & Therapy (2020) IF: 6.832
Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model

DOI: 10.1186/s13287-020-01733-4

PMID: 32456711
Sample: Plasma
BIOMACROMOLECULES (2021) IF: 6.988
Fabrication of In Situ Layered Hydrogel Scaffold for the Co-delivery of PGDF-BB/Chlorhexidine to Regulate Proinflammatory Cytokines, Growth Factors, and MMP-9 in a Diabetic Skin Defect Albino Rat Model

DOI: 10.1021/acs.biomac.0c01709

PMID: 33899465
Sample: Tissue homogenate,Serum
LIFE SCIENCES (2022) IF: 5.037
Integration of synthetic and natural derivatives revives the therapeutic potential of temozolomide against glioma- an in vitro and in vivo perspective

DOI: 10.1016/j.lfs.2022.120609

PMID: 35526592
Sample: C6 cell,U-87 MG cell,LN229 cell
RNA Biology (2019) IF: 5.477
MicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy in vitro and in vivo

DOI: 10.1080/15476286.2019.1657352

PMID: 31425004
Sample: Serum,Cell culture medium
Frontiers in Pharmacology (2021) IF: 5.811
Rivaroxaban Modulates TLR4/Myd88/NF-Kβ Signaling Pathway in a Dose-Dependent Manner With Suppression of Oxidative Stress and Inflammation in an Experimental Model of Depression

DOI: 10.3389/fphar.2021.715354

PMID: 34630092
Sample: hippocampal tissue
CELL TRANSPLANTATION (2022) IF: 4.064
Exosomes Derived From Mesenchymal Stem Cells Pretreated With Ischemic Rat Heart Extracts Promote Angiogenesis via the Delivery of DMBT1:

DOI: 10.1177/09636897221102898

Sample: heart tissue
ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.109
hUC-MSCs exosomal miR-451 alleviated acute lung injury by modulating macrophage M2 polarization via regulating MIF-PI3K-AKT signaling pathway

DOI: 10.1002/tox.23639

Sample: serum,NR8383 cell
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Investigation on the Q-markers of Bushen Huoxue Prescriptions for DR treatment based on chemometric methods and spectrum-effect relationship

DOI: 10.1016/j.jep.2021.114800

PMID: 34748867
Sample: eyes
Scientific Reports (2021) IF: 4.38
A nano phototheranostic approach of toluidine blue conjugated gold silver core shells mediated photodynamic therapy to treat diabetic foot ulcer

DOI: 10.1038/s41598-021-04008-x

PMID: 34961769
Sample: serum
JOURNAL OF CELL SCIENCE (2018) IF: 4.401
The effect of gap junction-mediated transfer of miR-200b on osteogenesis and angiogenesis in a co-culture of MSCs and HUVECs

DOI: 10.1242/jcs.216135

Sample: Tissue homogenate
EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 4.432
Amelioration of testosterone-induced benign prostatic hyperplasia using febuxostat in rats: The role of VEGF/TGFβ and iNOS/COX-2

DOI: 10.1016/j.ejphar.2020.173631

PMID: 33031799
Sample: Serum
International Journal of Nanomedicine (2019) IF: 4.471
Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

DOI: 10.2147/IJN.S184920

PMID: 30666115
Sample: Cell culture supernatant
INFLAMMOPHARMACOLOGY (2021) IF: 4.473
Quinone-rich fraction of Ardisia crispa (Thunb.) A. DC roots alters angiogenic cascade in collagen-induced arthritis

DOI: 10.1007/s10787-021-00816-9

PMID: 34091811
Sample: ankle
INFLAMMOPHARMACOLOGY (2022) IF: 4.473
Anti-inflammatory potential of liraglutide, a glucagon-like peptide-1 receptor agonist, in rats with peripheral acute inflammation

DOI: 10.1007/s10787-022-00978-0

PMID: 35412166
Sample: paw tissue
PLANTA MEDICA (2022) IF: 3.007
Curative effect and mechanisms of Radix Arnebiae oil on burn wound healing in rats

DOI: 10.1055/a-1997-5566

Sample: burn wound tissue
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2019) IF: 3.559
Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture

DOI: 10.1016/j.abb.2019.108108

PMID: 31550444
Sample: Cell lysate
Frontiers in Pediatrics (2022) IF: 3.569
Expression and Possible Role of Silent Mating Type Information Regulation 2 Homolog 1 in Post-necrotizing Enterocolitis Stricture in vivo and in vitro

DOI: 10.3389/fped.2022.836128

Sample: IEC-6 cell
LIFE SCIENCES (2020) IF: 3.647
Sphingosine kinase 1/sphingosine 1-phosphate/sphingosine 1-phosphate receptor 1 pathway: A novel target of geniposide to inhibit angiogenesis

DOI: 10.1016/j.lfs.2020.117988

Sample: Serum
COLLOIDS AND SURFACES B-BIOINTERFACES (2018) IF: 3.997
The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models

DOI: 10.1016/j.colsurfb.2018.04.060

Sample: VEGF solution after immobilization
HUMAN & EXPERIMENTAL TOXICOLOGY (2020) IF: 2.067
Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats:

DOI: 10.1177/0960327120960775

Sample: Tissue homogenate
HUMAN & EXPERIMENTAL TOXICOLOGY (2020) IF: 2.067
PTEN/PI3K/VEGF signaling pathway involved in the protective effect of xanthine oxidase inhibitor febuxostat against endometrial hyperplasia in rats:

DOI: 10.1177/0960327120914977

Sample: Tissue homogenate
Acupuncture in Medicine (2020) IF: 2.129
Electroacupuncture pretreatment promotes angiogenesis via hypoxia-inducible factor 1α and vascular endothelial growth factor in a rat model of chronic myocardial ischemia:

DOI: 10.1177/0964528420938378

Sample: Serum
Neural Regeneration Research (2018) IF: 2.234
Combination of mild therapeutic hypothermia and adipose-derived stem cells for ischemic brain injury

DOI: 10.4103/1673-5374.238617

Sample: serum
ACTA HISTOCHEMICA (2022) IF: 2.479
Potential stem cell—Conditioned medium and their derived exosomes versus omeprazole in treatment of experimental model of gastric ulcer

DOI: 10.1016/j.acthis.2022.151896

Sample: gastric tissue.
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
Molecular Mechanism of Traditional Chinese Ointment of Xuzhou Qufu Shengji in Infected Wounds

DOI: 10.1155/2022/4116563

PMID: 35069758
Sample: skin
Frontiers in Surgery (2021) IF: 2.718
The Effect of Waterpipe Tobacco Smoking on Bone Healing Following Femoral Fractures in Male Rats

DOI: 10.3389/fsurg.2021.722446

Sample: serum
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2022) IF: 2.723
Impact of repeated intravenous infusions of umbilical cord–derived versus bone marrow–derived mesenchymal stem cells on angiogenesis in a pregnant experimentally induced deep venous thrombosis rat model

DOI: 10.1007/s11626-022-00733-3

Sample: serum
Chinese Medicine (2020) IF: 2.96
Protective effects of essential oil from Fructus Alpiniae zerumbet on retinal Müller gliosis via the PPAR-γ-p-CREB signaling pathway

DOI: 10.1186/s13020-019-0283-4

PMID: 31938037
Sample: Serum,Cell culture medium
Cutaneous and Ocular Toxicology (2020) IF: 1.385
Comparison of the effect of topical bevacizumab and sorafenib in experimental corneal neovascularization

DOI: 10.1080/15569527.2020.1760877

Sample: corneal tissue
Experimental and Therapeutic Medicine (2019) IF: 1.448
MMP‑sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC‑mediated angiogenesis

DOI: 10.3892/etm.2019.7885

Sample: EPCs cells
JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
Citrus hystrix leaf extract attenuated diabetic‐cataract in STZ‐rats

DOI: 10.1111/jfbc.13258

Sample: serum
ANDROLOGIA (2019) IF: 1.84
Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM‐1 and VEGF‐A

DOI: 10.1111/and.13472

Sample: Serum
Medicinski Glasnik (2020)
The effect of bay leaf extract (Syzygium polyanthum) on vascular endothelial growth factor (VEGF) and CD31 (PECAM-1) expression in acute coronary syndrome.

DOI: 10.17392/1208-20

Sample: blood
World Journal of Pediatric Surgery (2022)
Expression and possible role of Smad3 in postnecrotizing enterocolitis stricture

DOI: 10.1136/wjps-2021-000289

Sample: IEC-6 cell
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2020) IF: 0.373
Effects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions

DOI: 10.1515/tjb-2019-0309

Sample: serum
Journal of Ocean University of China (2018) IF: 0.569
The Inhibitory Effects of PSS-Loaded Nanoparticles on the Dysfunction of Cardiac Microvascular Endothelia in Rats with Diabetic Cardiomyopathy

DOI: 10.1007/s11802-019-3998-z

Sample: Serum

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DOI: 10.1016/j.bioactmat.2021.03.020
PMID: 33842738	Sample: Cell culture supernatant

DOI: 10.1002/adfm.201706769
	Sample: neural stem cells (NSCs)

DOI: 10.1002/adhm.202201661
PMID: 36189833	Sample: BMSCs

DOI: 10.1016/j.biomaterials.2020.119985
	Sample: Cell culture supernatant

DOI: 10.1016/j.mtadv.2023.100349
	Sample: bone marrow mesenchymal stem cells (BMSCs)

DOI: 10.3390/biomedicines10050978
PMID: 35625715	Sample: liver tissue

DOI: 10.3390/ijms24032544
	Sample: retinal microvascular pericytes (RMPs)

DOI: 10.1155/2022/7319563
PMID: 35578728	Sample: brain

DOI: 10.1016/j.phymed.2022.154507
PMID: 36334391	Sample: serum

DOI: 10.1016/j.lfs.2022.121055
PMID: 36228770	Sample: serum

DOI: 10.1016/j.matdes.2022.110641
	Sample: EPCs

DOI: 10.1186/s13287-020-01733-4
PMID: 32456711	Sample: Plasma

DOI: 10.1021/acs.biomac.0c01709
PMID: 33899465	Sample: Tissue homogenate,Serum

DOI: 10.1016/j.lfs.2022.120609
PMID: 35526592	Sample: C6 cell,U-87 MG cell,LN229 cell

DOI: 10.1080/15476286.2019.1657352
PMID: 31425004	Sample: Serum,Cell culture medium

DOI: 10.3389/fphar.2021.715354
PMID: 34630092	Sample: hippocampal tissue

DOI: 10.1016/j.jep.2021.114800
PMID: 34748867	Sample: eyes

DOI: 10.1038/s41598-021-04008-x
PMID: 34961769	Sample: serum

DOI: 10.1016/j.ejphar.2020.173631
PMID: 33031799	Sample: Serum

DOI: 10.2147/IJN.S184920
PMID: 30666115	Sample: Cell culture supernatant

DOI: 10.1007/s10787-021-00816-9
PMID: 34091811	Sample: ankle

DOI: 10.1007/s10787-022-00978-0
PMID: 35412166	Sample: paw tissue

DOI: 10.1016/j.abb.2019.108108
PMID: 31550444	Sample: Cell lysate

DOI: 10.1016/j.colsurfb.2018.04.060
	Sample: VEGF solution after immobilization

Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

To Purchase E-EL-R2603

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Linearity

Target Information

Assay Procedures

Citations

Bioactive Materials (2021) IF: 14.593

ADVANCED FUNCTIONAL MATERIALS (2018) IF: 13.325

Advanced Fiber Materials (2022) IF: 12.958

Advanced Healthcare Materials (2022) IF: 11.092

BIOMATERIALS (2020) IF: 10.317

Materials Today Advances (2023) IF: 9.9179

MATERIALS & DESIGN (2022) IF: 7.991

Biomedicines (2022) IF: 6.081

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208

Oxidative Medicine and Cellular Longevity (2022) IF: 6.543

PHYTOMEDICINE (2022) IF: 6.656

LIFE SCIENCES (2022) IF: 6.78

Stem Cell Research & Therapy (2020) IF: 6.832

BIOMACROMOLECULES (2021) IF: 6.988

LIFE SCIENCES (2022) IF: 5.037

RNA Biology (2019) IF: 5.477

Frontiers in Pharmacology (2021) IF: 5.811

CELL TRANSPLANTATION (2022) IF: 4.064

ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.109

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Scientific Reports (2021) IF: 4.38

JOURNAL OF CELL SCIENCE (2018) IF: 4.401

EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 4.432

International Journal of Nanomedicine (2019) IF: 4.471

INFLAMMOPHARMACOLOGY (2021) IF: 4.473

INFLAMMOPHARMACOLOGY (2022) IF: 4.473

PLANTA MEDICA (2022) IF: 3.007

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2019) IF: 3.559

Frontiers in Pediatrics (2022) IF: 3.569

LIFE SCIENCES (2020) IF: 3.647

COLLOIDS AND SURFACES B-BIOINTERFACES (2018) IF: 3.997

HUMAN & EXPERIMENTAL TOXICOLOGY (2020) IF: 2.067

HUMAN & EXPERIMENTAL TOXICOLOGY (2020) IF: 2.067

Acupuncture in Medicine (2020) IF: 2.129

Neural Regeneration Research (2018) IF: 2.234

ACTA HISTOCHEMICA (2022) IF: 2.479

Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63

Frontiers in Surgery (2021) IF: 2.718

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2022) IF: 2.723

Chinese Medicine (2020) IF: 2.96

Cutaneous and Ocular Toxicology (2020) IF: 1.385

Experimental and Therapeutic Medicine (2019) IF: 1.448

JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662

ANDROLOGIA (2019) IF: 1.84

Medicinski Glasnik (2020)

World Journal of Pediatric Surgery (2022)

Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2020) IF: 0.373

Journal of Ocean University of China (2018) IF: 0.569

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DOI: 10.1177/0960327120960775
	Sample: Tissue homogenate

DOI: 10.1177/0960327120914977
	Sample: Tissue homogenate

DOI: 10.1016/j.acthis.2022.151896
	Sample: gastric tissue.

DOI: 10.1155/2022/4116563
PMID: 35069758	Sample: skin

DOI: 10.1186/s13020-019-0283-4
PMID: 31938037	Sample: Serum,Cell culture medium

DOI: 10.1080/15569527.2020.1760877
	Sample: corneal tissue