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Protein Purification and Separation

Affinity chromatography

2017-09-13Author:adminpraise:0

Affinity chromatography is a powerful chromatography technique of separating and purifying proteins based on the highly specific interaction between antigen and antibody, enzyme and substrate, or receptor and ligand. Such interactions including hydrogen bonding, ionic interaction, disulfide bridges, hydrophobic interaction, etc. Affinity chromatography are widely recognized for their high selectivity, high resolution and high capacity.


Cat. No.

Product name

Matrix

Binding capacity

E-CM-AF03

NHS Focurose 4FF

Highly cross-linked 4% agarose

16-23 μmol /mL (media)

E-CM-AF04

GST Focurose 4FF

Highly cross-linked 4% agarose

15-25mg (GST-tagged protein)/mL (medium)

E-CM-AF05

GST Focurose 4B

4% agarose

15-25mg (GST-tagged protein)/mL (media)

E-CM-AF06

Ni Focurose 6FF (TED)

Highly cross-linked 6% agarose

20-30 mg (His-tagged protein)/mL (medium)

E-CM-AF07

Ni Focurose 6FF (IMAC)

Highly cross-linked 6% agarose

45 mg (His-tagged protein)/mL (medium)

E-CM-AF08

Ni Focurose 6FF (IDA)

Highly cross-linked 4% agarose

40-50 mg (His-tagged protein)/mL (medium)

E-CM-AF09

Co Focurose 6FF (IMAC)

Highly cross-linked 6% agarose

45 mg (His-tagged protein)/mL (media)

E-CM-AF11

Protein A Focurose 4HF

Highly cross-linked 4% agarose and cellulose

~30 mg (Human IgG)/mL (media)

E-CM-AF12

Protein G Focurose 4FF

Highly cross-linked 4% agarose

~25 mg (Human IgG)/mL (media)

E-CM-AF13

Benzamidine Focurose 4FF(HS)

Highly cross-linked 4% agarose

25-50 mg (Trypsase)/mL (media)

E-CM-AF14

EAH Focurose 6FF

Highly cross-linked 6% agarose

0.4-0.5 mM Cl-/mL (media)

E-CM-AF15

Protein A Focurose 6FF

Highly cross-linked 6% agarose

0.4-0.6 mM Cl-/mL (media)

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