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Western Blot

Preparation of separating gel

2018-12-04Author:adminpraise:0

Components

Sample volume of each component corresponding to different separation gel volumes

5 mL

10 mL

15 mL

20 mL

25 mL

30 mL

40 mL

50 mL

6% Gel

 

H2O

2.6

5.3

7.9

10.6

13.2

15.9

21.2

26.5

30% Acrylamide

1.0

2.0

3.0

4.0

5.0

6.0

8.0

10.0

1.5M Tris-HCl(pH8.8)

1.3

2.5

3.8

5.0

6.3

7.5

10.0

12.5

10% SDS

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

10% (NH4)2S2O8

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

TEMED

0.004

0.008

0.012

0.016

0.02

0.024

0.032

0.04

8% Gel

 

H2O

2.3

4.6

6.9

9.3

11.5

13.9

18.5

23.2

30% Acrylamide

1.3

2.5

4.0

5.3

6.7

8.0

10.7

13.3

1.5M Tris-HCl(pH8.8)

1.3

2.5

3.8

5.0

6.3

7.5

10.0

12.5

10% SDS

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

10% (NH4)2S2O8

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

TEMED

0.003

0.006

0.009

0.012

0.015

0.018

0.024

0.03

10% Gel

 

H2O

1.9

4.0

5.9

7.9

9.9

11.9

15.9

19.8

30% Acrylamide

1.7

3.3

5.0

6.7

8.3

10.0

13.3

16.7

1.5M Tris-HCl(pH8.8)

1.3

2.5

3.8

5.0

6.3

7.5

10.0

12.5

10% SDS

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

10% (NH4)2S2O8

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

TEMED

0.002

0.004

0.006

0.008

0.01

0.012

0.016

0.02

12% Gel

 

H2O

1.6

3.3

4.9

6.6

8.2

9.9

15.9

16.5

30% Acrylamide

2.0

4.0

6.0

8.0

10.0

12.0

13.3

20.0

1.5M Tris-HCl(pH8.8)

1.3

2.5

3.8

5.0

6.3

7.5

10.0

12.5

10% SDS

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

10% (NH4)2S2O8

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

TEMED

0.002

0.004

0.006

0.008

0.01

0.012

0.016

0.02

15% Gel

 

H2O

1.1

2.3

3.4

4.6

5.7

6.9

9.2

11.5

30% Acrylamide

2.5

5.0

7.5

10.0

12.5

15.0

20.0

25.0

1.5M Tris-HCl(pH8.8)

1.3

2.5

3.8

5.0

6.3

7.5

10.0

12.5

10% SDS

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

10% (NH4)2S2O8

0.05

0.1

0.15

0.2

0.25

0.3

0.4

0.5

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