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Chromatographic Media

Chromatography is a most frequently used laboratory technique for separation and purification of biomolecules mixture. The mixture is dissolved in the mobile phase (a kind of fluid), which carries it through the stationary phase (a structure holding another material). The separation is based on differential partitioning between the mobile and stationary phases.

According to the different separation mechanism and principle, chromatography techniques are classified into different types for separation and purification process of the various target biomolecules. There are 4 types of commonly used chromatography techniques: Affinity chromatography, Ion exchange chromatography (IEC), Gel filtration (also known as Size-exclusion chromatography (SEC) and molecular sieve chromatography), and Hydrophobic interaction chromatography (HIC). 

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