Detection of T/B/NK (4-color) in C57BL/6 mouse peripheral blood

Tips:

1.  Add CD45 indicators to peripheral blood samples, the lymphocyte populations can be gated directly through CD45 and SSC.

2.  The CD3/CD4/CD8 cell populations are obvious, it can effectively distinguish between positive and negative cells even without Isotype Control.

3.  The detection indicators of NK cells should be selected based on different mouse varieties, usually C57BL/6 mouse use NK1.1, and BALB/c mouse use CD49b (DX5). CD3-NK1.1⁺/CD3^-CD49b⁺ is NK cells.