Detection of Lymphocyte (4 colors) in Mouse Tumor - Elabscience
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Detection of Lymphocyte (4 colors) in Mouse Tumor


Purpose

Sample

Antibody Collocation

Adjust the voltage

1

Blank

Adjust compensation

2

CD45-PerCP/Cyanine5.5

3

CD3-FITC

4

CD4-PE

5

CD8-APC

Full panel

6

CD45-PerCP/Cyanine5.5, CD3-FITC, CD4-PE, CD8a-APC

Marker

Fluorochrome

Clone No.

Cat. No.

CD45

PerCP/Cyanine5.5

30-F11

E-AB-F1136J

CD3

FITC

17A2

E-AB-F1013C

CD4

PE

GK1.5

E-AB-F1097D

CD8a

APC

53-6.7

E-AB-F1104E


Tips:

1. In tumor tissue, the majority cells are tumor cells, and the proportion of lymphocytes is relatively low. CD45 and SSC can be used to gate the lymphocytes.

2. The lymphocyte gate in tumor cells is defined as the gate of CD45high and SSClow on the CD45/SSC scatter plot.




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