Detection of Treg (3-color) in Mouse Spleen

Tips:

1. Mouse Treg marker is CD4⁺ CD25⁺ Foxp3⁺.

2. CD4 cell population is obvious, and there is no need of Isotype Control. But CD25 and Foxp3 populations are not obvious, and Isotype Controls are needed.

3. There is fluorescence spillover, so it is necessary to set single positive tubes for compensation.

4. Please be careful that inappropriate use of Fixation/Permeabilization buffer may cause high background and unclear cell clustering.