Your Essential Handbook for Cellular Energy Metabolism Studies
● Systematically covers key metabolic pathways including glycolysis, fatty acid β-oxidation, TCA cycle, electron transport chain, oxidative phosphorylation and amino acid metabolism, with comprehensive mitochondrial metabolism pathway diagrams.
● Provides standardized protocols for major metabolic assays, including glucose uptake, OCR, ECAR, FAO and ATP/ADP ratio detection in cell lines, primary immune cells and tissue samples.
● Features immune cell-specific metabolic workflows for polarized macrophages (M1/M2) and T cell subsets, including naïve, effector, memory, Treg and CAR-T cells.
● Includes validated assay data, troubleshooting tips, microplate reader settings and comparisons between fluorescence-based detection and Seahorse analysis.
● Features high-impact journal research cases, metabolism assay solutions and practical assay operation videos to support efficient experimental design and application.
Trusted by global researchers, this handbook serves students, postdocs and scientists studying immunometabolism, cancer metabolism, mitochondrial biology, metabolic diseases, preclinical pharmacology and cell therapy.
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