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FAQs

FAQs for ELISA

2017-05-17Author:adminpraise:1

 Frequently Asked Questions    (Quesation       Answer


 Instrumentation

Do I have to use a wavelength correction?

 Reading at dual wavelengths is to correct the optical density contributed by the plate wells and other nonspecific interference. Our plates are chosen for their optical quality; therefore this correction is actually very small. If the dual wavelength feature is not available, data read at the recommended detection wavelength should be little affected.  

Can I use a fully automated microplate ELISA analyzer to do the assay?

 Normally, the amount and ratio of each reagent a fully automated microplate ELISA analyzer needs are not the same as in our kit, so it’s not applicable. Whereas if you can tell the amount of each reagent according to the machine manual and be sure the machine can be set the same procedure as in our manual, we receive special order. The price is based on your demand.

 Sample and Species  

What species does your kit work with?

Kits where the molecule is conserved among all species (e.g. testosterone, cortisol) are species independent, and may be used with any species. Kits that list a specific species in the name (e.g. human) are specific for that species. If the species is not specified in the manual, the product is species independent. The information may also be found on the product specific page on our website.

How do I know if I need to dilute or extract my samples?

If the analyte level in your sample is out of the upper limit of the assay, you may dilute your sample and run directly with the kit. If your analyte level is out of the lower limit of the assay, extraction and concentration is necessary, or you need find a kit with higher sensitivity.

  Data Analysis

Why are my ODs different than those shown in the instruction manual?

It is normal to see a small variation in OD values between experiments. The variation is closely related to operation skills of the operators and the experimental environments. The standard curve in the manual is just for reference. As long as the OD values are with good gradient, the values are valid.

What is the best way to analyze my data? 

For the most accurate results we recommend that 4 parameter logistic (4-PL) curve fitting software be used. The data may be near-linear by plotting the log of concentrations versus the log of OD values. By choosing a better curve fit for your data, you will ultimately have more accurate returned sample values. If this kind of standard curve is unavailable in the software, user can fit a curve which is chose by software automatically.