293T [HEK-293T] Cell Line

Name: 293T [HEK-293T] Cell Line
Brand: Elabscience
SKU: EP-CL-0005
Availability: InStock

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Cell Name	293T [HEK-293T] Cell Line
Organism	Homo sapiens, Human
Age	Fetus
Tissue	Embryonic kidney
Morphology	Epithelial
Growth Properties	Adherent
Descriptions	This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses. It has been widely used for retroviral production, gene expression and protein production.
Biosafety Level	2

Complete Growth Medium	DMEM(EP-CM-L0031)+10% FBS+1% P/S(EP-CM-L0048) (293T [HEK-293T] Cell Line complete medium (EP-ML-0005) is recommended.)
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1:3-1:6
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

EP-CM-P0031	DMEM (High Glucose), powder
EP-CM-L0031	DMEM (High glucose)
EP-CM-L0048	Penicillin-Streptomycin Solution (100×)
EP-CM-L0006	Phosphate Buffer (PBS)
EP-CM-L0060	200mM L-Alanyl-L-Glutamine Solution (100×)
EP-CM-L0043	0.25% Trypsin Solution

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