293T [HEK-293T] Cell Line

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      Catalog number:EP-CL-0005

      Size:
      • 1×10⁶ cells/vial
      Qty:
      - +
      Price: $401

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      Origin and General Characteristics

      Cell Name 293T [HEK-293T] Cell Line
      Organism Homo sapiens, Human
      Age Fetus
      Tissue Embryonic kidney
      Morphology Epithelial
      Growth Properties Adherent
      Descriptions This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses. It has been widely used for retroviral production, gene expression and protein production.
      Biosafety Level 2

      Culture Conditions and Handling

      Complete Growth Medium DMEM +10% FBS +1% P/S
      Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
      Subcultivation Ratio 1:3-1:6
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
      Storage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%
      Temperature: 37℃

      Related Resources

      Recommendations for handling of cryopreserved cells

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