2-step plus Poly-HRP Anti Rabbit/Mouse IgG Detection System(with DAB Solution)
Price: $ 400
Price: $ 180
Price: $ 100
Size:
18mL
6mL
3mL
Quantity:
- Applications: IHC
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For research use only. Order now, ship in 3 days
Product Details
Product Introduction | 2-step plus is a two-step immunohistochemical broad spectrum detection reagent. It polymerizes monovalent Fab fragments of secondary antibody and enzyme, which replaces the secondary antibody and tertiary antibody in traditional method, can directly amplify the binding signal of antibody-antigen. This method not only retains the specific binding ability of antibody with antigen, but also can effectively avoid space steric hindrance caused by excessive polymer molecules. Compared with the traditional SP three-step method, this kit has the characteristics of simple, rapid, high-sensitivity. This system abandons the using of biotin, so it can avoid background staining by endogenous biotin. It can be used in IHC, in which the primary antibody is monoclonal/polyclonal antibody derived from rabbit/mouse. The unique polymer auxiliary agent for macromolecular detection system can detect the binding primary antibody better. Diluent for DAB concentrated solution has been provided in this kit to avoid the influence of the different water acidity and alkalinity on the DAB Chromogenic Agent. |
Application | IHC |
Storage | Store at 2~8°C, shading light. Avoid of freezing. Valid for 12 months. The reagents are valid within 6 months after opening. |
Shipping | Biological ice pack at 4 ℃ |
Cautions |
1. This product couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence. 2. For your safety and health, please wear the lab coat and disposable gloves before the experiments. |
Citations
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TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile
IF:41.584
Journal:CELL
DOI:10.1016/j.cell.2022.02.010
PMID:35303428
-
Paeniclostridium sordellii hemorrhagic toxin targets TMPRSS2 to induce colonic epithelial lesions
IF:17.694
Journal:Nature Communications
DOI:10.1038/s41467-022-31994-x
PMID:35882856
-
Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical Evaluation
IF:6.706
Journal:Nutrients
-
GLP-1 Mediates Regulation of Colonic ACE2 Expression by the Bile Acid Receptor GPBAR1 in Inflammation
IF:6.6
Journal:Cells
PMID:35406751
-
Discovery of a AHR pelargonidin agonist that counter-regulates Ace2 expression and attenuates ACE2-SARS-CoV-2 interaction
IF:5.858
Journal:BIOCHEMICAL PHARMACOLOGY
PMID:33872570
-
A recombinant glucocorticoid-induced leucine zipper protein ameliorates symptoms of dextran sulfate sodium-induced colitis by improving intestinal permeability
IF:5.192
Journal:FASEB JOURNAL
PMID:34613638
-
LRIG2 regulates cell proliferation, migration and apoptosis of osteosarcoma
IF:4.638
Journal:BMC CANCER
DOI:10.1186/s12885-022-10123-3
PMID:36183058
-
Arsenic-induced uterine apoptotic damage is protected by ethyl acetate fraction of Camellia sinensis (green tea) via Bcl-2-BAX through NF-κB regulations in Wistar rats
IF:4.223
Journal:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
DOI:10.1007/s11356-021-13457-w
PMID:33774797
-
Assessment of the cellular integrity and expression of melatonin receptor (MTNR1A) in the retina assaulted by ethanol and acetaminophen
IF:3.247
Journal:HUMAN & EXPERIMENTAL TOXICOLOGY
-
circTUBGCP5 promotes liver cancer progression and glycolysis by up-regulating the expression of ACSL4
IF:3.206
Journal:Applied Biological Chemistry
Experiment Operation Video
Protocols
• Western Blot Guide
• Immunohistochemistry Guide for Slide-mounted Paraffin Sections
• Immunofluorescence Guide
• Immunohistochemistry Troubleshooting Tips
• Western Blot Troubleshooting Tips
• Immunofluorescence Troubleshooting Tips
• Comparison of Protein Separation Linear Range
• ImmunoPrecipitation Protocol