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5’-Nucleotidase (5'-NT) Activity Assay Kit

Cat.No.:E-BC-K196-M
Detection instrument: Microplate reader (550 nm)

Detection method: Colorimetric method

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Detection principle

5'-Nucleotide enzyme (5'-NT, EC 3.1.3.5), full name is 5'-ribonucleotide phosphohydrolase, is a special phosphohydrolase that specifically hydrolyzes 5'-phosphoric acid attached to pentose in 5'-nucleotide. This enzyme is widely distributed in the cell membrane of various tissues of human and animal. Only the 5'-NT released by the tissue cells of the hepatobiliary system may enter the blood. Therefore, the source of serum 5'-NT has certain specificity, and the determination of serum 5'-NT has important value for the diagnosis of hepatobiliary diseases.

Performance characteristics

Synonyms	5’-NT
Sample type	Serum, plasma, animal tissue
Sensitivity	28.0 U/L
Detection range	28.0-581 U/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 6%Average intra-assay CV: 3.300%
Other instruments required	Pipettor, Water bath, Centrifuge
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

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Collection and treatment of common samples for metabolism assay detection

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