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CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Cat:E-BC-K022-M Citations (22)
Manual MSDS

Price: $ 280

Price: $ 200

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;urine;cells;cell culture supernatant;tissue
  • Detection Instrument: Microplate reader(530-570 nm,optimum wavelength: 550 nm)
  • Reasearch Areas: Oxidative stress
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Product Details
Detection Principle Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
Synonyms CuZn-SOD;Mn-SOD;T-SOD
Sample Type serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 1.35 U/mL
Detection Range 1.35-62 U/mL
Detection Method Colorimetric method
Assay Time 70 min
Precision inter-assay CV: 9.6% | intra-assay CV: 5.1%
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Mouse serum 5-10
Rat serum 6-15
Urine 2-3
Human hydrothorax 2-3
10% Mouse liver tissue homogenate 100-200
10% Mouse brain tissue homogenate 20-30
10% Mouse kidney tissue homogenate 50-120
10% Rat kidney tissue homogenate 50-120
HepG2 cells (5.21mgprot/mL) 15-25
Cell culture supernatant 1
The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.