CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
Price: $ 280
Price: $ 200
Size:
96T
48T
Quantity:
- Sample Type: serum;plasma;urine;cells;cell culture supernatant;tissue
- Detection Instrument: Microplate reader(530-570 nm,optimum wavelength: 550 nm)
- Reasearch Areas: Oxidative stress
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Product Details
Detection Principle | Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not. | ||||||||||||||||||||||
Synonyms | CuZn-SOD;Mn-SOD;T-SOD | ||||||||||||||||||||||
Sample Type | serum,plasma,urine,cells,cell culture supernatant,tissue | ||||||||||||||||||||||
Sensitivity | 1.35 U/mL | ||||||||||||||||||||||
Detection Range | 1.35-62 U/mL | ||||||||||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||||||||||
Assay Time | 70 min | ||||||||||||||||||||||
Precision | inter-assay CV: 9.6% | intra-assay CV: 5.1% | ||||||||||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl) | ||||||||||||||||||||||
Storage | This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. | ||||||||||||||||||||||
Valid Period | 12 months | ||||||||||||||||||||||
Images | |||||||||||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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