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(KO Validated) HSPB1 Polyclonal Antibody

Cat:E-AB-66764
Manual MSDS
  • +1

Price: $ 580

Price: $ 360

Price: $ 220

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IF
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:HeLa
Verified Samples in IF:HeLa
Dilution

WB 1:500-1:2000, IF 1:50-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant fusion protein of human HSPB1
Abbre HSPB1
Synonyms HSPB1;CMT2F;HEL-S-102;HMN2B;HS.76067;HSP27;HSP28;Hsp25;SRP27
Swissprot
Calculated MW 22kDa
Observed MW 27KDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm,Nucleus,cytoskeleton,spindle
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Purification Method Affinity purification
Research Areas Cancer;Cardiovascular;Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).