A549 [A-549] Cell Line

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      Catalog number:EP-CL-0016

      Size:
      • 1×10⁶ cells/vial
      Qty:
      - +
      Price: $302

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      Origin and General Characteristics

      Cell Name A549 [A-549] Cell Line
      Organism Homo sapiens, Human
      Age 58 years
      Tissue Lung
      Morphology Epithelial
      Growth Properties Adherent
      Descriptions Studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway.
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium Ham's F-12K +10% FBS +1% P/S
      Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
      Subcultivation Ratio 1:3-1:6
      Medium Renewal 2 to 3 times per week
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
      Storage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%
      Temperature: 37℃

      Special Features of the Cell Line

      Antigen Expression The cells are positive for keratin by immunoperoxidase staining.
      Applications Transfection host.

      Related Resources

      Recommendations for handling of cryopreserved cells

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