Acid Phosphatase (ACP) Activity Assay Kit
Price: $ 150
Price: $ 150
Size:
100Assays
50Assays
Quantity:
- Sample Type: serum;plasma;urine;tissue;cells
- Detection Instrument: Spectrophotometer(520 nm)
- Reasearch Areas: Amino acids proteins
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Product Details
Detection Principle | Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm. | ||||||||||||||||||
Synonyms | ACP | ||||||||||||||||||
Sample Type | serum,plasma,urine,tissue,cells | ||||||||||||||||||
Sensitivity | 0.27 U/100 mL | ||||||||||||||||||
Detection Range | 0.27-40 U/100 mL | ||||||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||||||
Assay Time | 70 min | ||||||||||||||||||
Precision | inter-assay CV: 7.1% | intra-assay CV: 2.8% | ||||||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) | ||||||||||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||||||||||
Valid Period | 12 months | ||||||||||||||||||
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Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |