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Acid Phosphatase (ACP) Activity Assay Kit

Cat:E-BC-K094-S
Manual MSDS

Price: $ 150

Price: $ 150

Size:
100Assays 50Assays
Quantity:
  • Sample Type: serum;plasma;urine;tissue;cells
  • Detection Instrument: Spectrophotometer(520 nm)
  • Reasearch Areas: Amino acids proteins
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Product Details
Detection Principle Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
Synonyms ACP
Sample Type serum,plasma,urine,tissue,cells
Sensitivity 0.27 U/100 mL
Detection Range 0.27-40 U/100 mL
Detection Method Colorimetric method
Assay Time 70 min
Precision inter-assay CV: 7.1% | intra-assay CV: 2.8%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human milk 1
Human saliva 1
Human urine 1
Human serum 1
HepG2 cells homogenization 2-8
10% Mouse kidney tissue homogenization 8-12
10% Mouse liver tissue homogenization 8-12
10% Mouse spleen tissue homogenization 8-12
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.