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Cat.No.:E-AB-F11260
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Alternate Names | T6, R4,CD 1a,CD1A ,T-cell surface glycoprotein CD1a |
Swissprot | P06126 |
References |
1. Blumberg RS, et al. 1995. Immunol. Rev. 147:5.
2. Calabi F, et al. 1991. Tissue Antigens 37:1. 3. Melian A, et al. 1996. Curr. Opin. Immunol. 8:82. |
Form | Liquid |
Clone No. | OKT-6(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | AF/LE Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F097930] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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