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AF/LE Purified Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]

Uniprot : Q08722,Q61735,P97829

Cat.No.:E-AB-F10160
Applications: FCM
Isotype: Mouse IgG1, κ
Host: Mouse

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Antigen Details

Alternate Names	Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP
Swissprot	Q08722,Q61735,P97829
References	1. Gresham HD, Dale BM, Potter JW, et al. J Exp Med. 2000; 191(3):515-528. 2. Jiang P, Lagenaur CF, Narayanan V. J Biol Chem. 1999; 274(2):559-562. 3. Lindberg FP, Bullard DC, Caver TE, Gresham HD, Beaudet AL, Brown EJ. Science. 1996; 274(5288):795-798. 4. Lindberg FP, Gresham HD, Schwarz E, Brown EJ. . J Cell Biol. 1993; 123(2):485-496. 5. Mi ZP, Jiang P, Weng WL, Lindberg FP, Narayanan V, Lagenaur CF. J Comp Neurol. 2000; 416(3):335-344. 6. Oldenborg PA, Zheleznyak A, Fang YF, Lagenaur CF, Gresham HD, Lindberg FP. Science. 2000; 288(5473):2051-2054. 7. Reinhold MI, Lindberg FP, Plas D, Reynolds S, Peters MG, Brown EJ. J Cell Sci. 1995 November; 108(Pt 11):3419-3425.

Product Details

Form	Liquid
Clone No.	MIAP410(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	AF/LE Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F097930]
Reactivity	Human,Mouse,Rat
Application	FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
Biotin Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
FITC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	20Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
PE Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
APC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
PerCP Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]	25μg, 100μg	Check More

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