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AF/LE Purified Anti-Mouse CD122/IL-2RB Antibody[5H4]

Uniprot : P16297

Cat.No.:E-AB-F10290
Applications: FCM
Isotype: Rat IgG2a, κ
Host: Rat

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Antigen Details

Alternate Names	Interleukin-2 receptor subunit beta,Il2rb,IL-2 receptor subunit beta, IL-2R subunit beta,IL-2RB,High affinity IL-2 receptor subunit beta,p70-75,CD122
Swissprot	P16297
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245. 3. Suzuki H, et al. 1995. Science 268:1472. 4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Product Details

Form	Liquid
Clone No.	5H4(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
Biotin Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
FITC Anti-Mouse CD122/IL-2RB Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD122/IL-2RB Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD122/IL-2RB Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD122/IL-2RB Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD122/IL-2RB Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD122/IL-2RB Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD122/IL-2RB Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD122/IL-2RB Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
PE Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
APC Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD122/IL-2RB Antibody[5H4]	25μg, 100μg	Check More

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