AF/LE Purified Anti-Mouse CD29 Antibody[KMI6] E-AB-F09980

Uniprot : P09055
  • Host: Rat
  • Applications: FCM
  • Isotype: Rat IgG2a, κ

To Purchase E-AB-F09980

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  • 50 μg
  • 500 μg
  • 1 mg
Price: $75
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Antigen Details

Alternate Names Integrin beta-1,ITB1,Itgb1,Fibronectin receptor subunit beta,VLA-4 subunit beta,CD29
Swissprot P09055
References
1. Jackson Laboratory. Mouse Genome Database. 1998, Sept. 17.
2. Jacobsen K, Miyake K, Kincade PW, Osmond DG. J Exp Med. 1992; 176(4):927-935.
3. Springer TA. Nature. 1990; 346(6283):425-434.
4. Wadsworth SA, Chang AC, Hong MJ, Halvorson MJ, Otto S, Coligan JE. J Immunol. 1995; 154(5):2125-2133.
5. Wu X, Miyake K, Medina KL, Kincade PW, Gimble JM. Hybridoma. 1994; 13(5):409-416.

Product Details

Form Liquid
Clone No. KMI6(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

The KMI6 antibody reacts with the 130-kDa integrin ß1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin achains. With a1 through a6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with av (CD51), it forms avß1 integrin. It also associates with the integrin a7, a8, and a9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, smooth muscle, epithelia, and oocytes. The epitope recognized by the KMI6 mAb can be detected by immunoblot in unreduced extracts from a variety of cells and tissues having a number of different ß1-integrin heterodimer combinations. However, the epitope is not well expressed on the surface of the majority of hematopoietic cells. It is detected on the surface of fetal liver cells, some bone marrow stromal cells, and some cultured hematopoietic cell lines, including P815 and PA6. Surface expression of the CD29 epitope recognized by mAb KMI6 on thymocytes and T lymphocytes appears to be developmentally regulated, and KMI6 antibody enhances the adhesion of KMI6 thymocytes to fibronectin and laminin.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F09980 AF/LE Purified Anti-Mouse CD29 Antibody[KMI6] E-AB-F09980 50 μg, 500 μg, 1 mg
E-AB-F0998A Purified Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998A 50 μg, 100 μg
E-AB-F0998B Biotin Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998B 50 μg, 100 μg
E-AB-F0998C FITC Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998C 50 Tests, 100 Tests, 500 Tests
E-AB-F0998E APC Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998E 50 Tests, 100 Tests, 500 Tests
E-AB-F0998L AF488 Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998L 20 Tests, 50 Tests, 100 Tests, 500 Tests
E-AB-F0998M AF647 Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998M 20 Tests, 50 Tests, 100 Tests, 500 Tests
E-AB-F0998UC FITC Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998UC 25μg, 50μg, 100μg
E-AB-F0998UE APC Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998UE 25μg, 50μg, 100μg
E-AB-F0998UL AF488 Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998UL 25μg, 50μg, 100μg
E-AB-F0998UM AF647 Anti-Mouse CD29 Antibody[KMI6] E-AB-F0998UM 25μg, 50μg, 100μg

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