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AF/LE Purified Anti-Mouse CD366/Tim-3 Antibody[RMT3-23]

Uniprot : Q8VIM0

Cat.No.:E-AB-F11920
Applications: FCM,Block
Isotype: Rat IgG2a, κ
Host: Rat

Applications:

FCM,Block

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500μg
1mg

Price: $95

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Antigen Details

Alternate Names	TIM3, TIMD3,HAVcr-2,TIMD-3
Swissprot	Q8VIM0
References	1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699. 2. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 3. Wang F, et al. 2009. Immunobiology 214:342.

Product Details

Form	Liquid
Clone No.	RMT3-23(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity	Mouse
Application	FCM,Block (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD366/Tim-3 Antibody[RMT3-23]	25μg, 100μg	Check More
PE Anti-Mouse CD366/Tim-3 Antibody[RMT3-23]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD366/Tim-3 Antibody[RMT3-23]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD366/Tim-3 Antibody[RMT3-23]	25μg, 100μg	Check More
APC Anti-Mouse CD366/Tim-3 Antibody[RMT3-23]	25μg, 100μg	Check More

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