AF/LE Purified Anti-Rat CD8a Antibody[OX-8] E-AB-F10980

Uniprot : P07725
  • Host: Mouse
  • Applications: FCM,Wb,IHC,IP
  • Isotype: Mouse IgG1, κ

To Purchase E-AB-F10980

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $75
Qty:

Antigen Details

Alternate Names T-cell surface glycoprotein CD8 alpha chain,CD8A,T-lymphocyte differentiation antigen T8/Leu-2,MAL
Swissprot P07725
References
1. Johnson P, et al. 1985. EMBO J. 4:2539.
2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.

Product Details

Form Liquid
Clone No. OX-8(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control AF/LE Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F097930]
Reactivity Rat
Application FCM,Wb,IHC,IP (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F10980 AF/LE Purified Anti-Rat CD8a Antibody[OX-8] E-AB-F10980 50 μg, 500 μg, 1 mg
E-AB-F1098A Purified Anti-Rat CD8a Antibody[OX-8] E-AB-F1098A 50 μg, 100 μg
E-AB-F1098B Biotin Anti-Rat CD8a Antibody[OX-8] E-AB-F1098B 50 μg, 100 μg
E-AB-F1098C FITC Anti-Rat CD8a Antibody[OX-8] E-AB-F1098C 100 Tests, 200 Tests, 500 Tests
E-AB-F1098D PE Anti-Rat CD8a Antibody[OX-8] E-AB-F1098D 100 Tests, 200 Tests, 500 Tests
E-AB-F1098E APC Anti-Rat CD8a Antibody[OX-8] E-AB-F1098E 100 Tests, 200 Tests, 500 Tests
E-AB-F1098F PerCP Anti-Rat CD8a Antibody[OX-8] E-AB-F1098F 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1098G PE/Cyanine5 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098G 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1098H PE/Cyanine7 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098H 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1098I PE/Cyanine5.5 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098I 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1098J PerCP/Cyanine5.5 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098J 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1098L AF488 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098L 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1098M AF647 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098M 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1098UC FITC Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UC 25μg, 50μg, 100μg
E-AB-F1098UD PE Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UD 25μg, 50μg, 100μg
E-AB-F1098UE APC Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UE 25μg, 50μg, 100μg
E-AB-F1098UF PerCP Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UF 25μg, 50μg, 100μg
E-AB-F1098UG PE/Cyanine5 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UG 25μg, 50μg, 100μg
E-AB-F1098UH PE/Cyanine7 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UH 25μg, 50μg, 100μg
E-AB-F1098UI PE/Cyanine5.5 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UI 25μg, 50μg, 100μg
E-AB-F1098UJ PerCP/Cyanine5.5 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UJ 25μg, 50μg, 100μg
E-AB-F1098UL AF488 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UL 25μg, 50μg, 100μg
E-AB-F1098UM AF647 Anti-Rat CD8a Antibody[OX-8] E-AB-F1098UM 25μg, 50μg, 100μg

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