Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Performance characteristics

Synonyms	ALP
Sample type	Serum,plasma,tissue,cells
Sensitivity	0.13 King unit/100 mL
Detection range	0.13-50 King unit/100 mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 8.500%Average intra-assay CV: 5.100%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.13-50 King unit/100 mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
Human urine	1
Rat serum	1
Cells culture supernatant	1
10% Mouse kidney tissue homogenate	30-50
0% Mouse liver tissue homogenate	1
10% Mouse brain tissue homogenate	1
HepG2 cells	1

Note: The diluent is normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Science Advances (2019) IF: 14.143
A prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells

DOI: 10.1126/sciadv.aax0064

Sample: serum
ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022) IF: 9.028
Lipid Metabolic Disorder Induced by Pyrethroids in Nonalcoholic Fatty Liver Disease of Xenopus laevis

DOI: 10.1021/acs.est.2c00516

Sample: plasma
JOURNAL OF HAZARDOUS MATERIALS (2020) IF: 9.038
The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis

DOI: 10.1016/j.jhazmat.2020.124876

PMID: 33360192
JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
BMSC exosome-enriched acellular fish scale scaffolds promote bone regeneration

DOI: 10.1186/s12951-022-01646-9

PMID: 36224596
Sample: BMSCs
BIOCHEMICAL PHARMACOLOGY (2022) IF: 6.1
Pterostilbene-isothiocyanate impedes RANK/TRAF6 interaction to inhibit osteoclastogenesis, promoting osteogenesis in vitro and alleviating glucocorticoid induced osteoporosis in rats

DOI: 10.1016/j.bcp.2022.115284

PMID: 36209841
Sample: serum,MC3T3-E1 cells,hFOB 1.19 cells
JOURNAL OF CELLULAR PHYSIOLOGY (2022) IF: 6.513
IGF2BP2, an RNA-binding protein regulates cell proliferation and osteogenic differentiation by stabilizing SRF mRNA

DOI: 10.1002/jcp.30919
Frontiers in Endocrinology (2021) IF: 5.555
Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism

DOI: 10.3389/fendo.2021.759843

PMID: 34777254
Sample: serum
Frontiers in Pharmacology (2022) IF: 5.811
Uncovering the Pharmacological Mechanisms of Gexia-Zhuyu Formula (GXZY) in Treating Liver Cirrhosis by an Integrative Pharmacology Strategy.

DOI: 10.3389/fphar.2022.793888

Sample: serum
Frontiers in Pharmacology (2021) IF: 5.811
Phillygenin Attenuates Carbon Tetrachloride-Induced Liver Fibrosis via Modulating Inflammation and Gut Microbiota

DOI: 10.3389/fphar.2021.756924

PMID: 34621179
Sample: serum
RARE METALS (2021) IF: 4.003
Degradation and biological performance of porous osteomimetic biphasic calcium phosphate in vitro and in vivo

DOI: 10.1007/s12598-021-01814-0

Sample: MG63 cell
BONE (2021) IF: 4.398
Coupling Induction of Osteogenesis and Type H Vessels by Pulsed Electromagnetic Fields in Ovariectomy-induced Osteoporosis in Mice

DOI: 10.1016/j.bone.2021.116211

PMID: 34560308
Sample: serum
PLoS Neglected Tropical Diseases (2023) IF: 4.781
Improvement of Antialveolar echinococcosis efficacy of novel Albendazole-Bile acids Derivatives with Enhanced Oral Bioavailability

DOI: 10.1371/journal.pntd.0011031
Scientific Reports (2022) IF: 4.996
Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

DOI: 10.1038/s41598-022-21816-x

Sample: serum
NEUROSCIENCE LETTERS (2021) IF: 3.046
Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

DOI: 10.1016/j.neulet.2021.136385

PMID: 34871743
Sample: serum
BMC MUSCULOSKELETAL DISORDERS (2022) IF: 2.562
miR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP

DOI: 10.1186/s12891-022-05731-w

PMID: 35978328
Sample: hBMSC
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540

Sample: hepatic tissue,serum
Molecular Medicine Reports (2020) IF: 2.952
Mechanisms by which electroacupuncture‑mediated histone acetylation mitigates bone loss in rats with ovariectomy‑induced osteoporosis

DOI: 10.3892/mmr.2020.11430

PMID: 32945471
Sample: Serum
Toxicology Reports (2022)
Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

DOI: 10.1016/j.toxrep.2022.06.005

Sample: plasma
Toxicology Reports (2017)
Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

DOI: 10.1016/j.toxrep.2017.12.002

Sample: plasma

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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Oct 09 2022 ]

Asked: How to convert King Unit /100 mL to U/L?

adminSubmitted [ Oct 09 2022 ]

Answered: King Unit /100 mL = 7.14 U/L

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