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Annexin V-AF647 Azide-Free Lyophilized Powder[113U]

  • Cat.No.:E-CK-A113U

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Introduction

Elabscience® Annexin V-AF647 Azide-Free Lyophilized Powder is developed for long-term storage and user-defined concentrations of AF647-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The AF647-conjugated format of this protein, Annexin V-AF647 binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V-AF647 can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.

Components

Cat. Products 25 ug 50 ug 100 ug 200 ug Storage
E-CK-A113U Annexin V-AF647 Azide-Free Lyophilized Powder[113U] 25 μg 25 μg × 2 100 μg 100 μg × 2 -20°C
Manual One Copy

Jurkat cells were treated with 5 μM Camptothecin and detected with this reagent and DAPI.

Jurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-AF647 single-positive cells were early apoptotic cells, Annexin V-AF647 and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.

Reconstitution

Reconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg/mL. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg/mL Annexin V-AF647 have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin V-AF647. Otherwise, the lyophilized cake may not be thoroughly soaked in water.

Storage

The lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival. 
After reconstitution, the regent can be stored in the dark under sterile conditions at 2~8°C for at least 6 months. 

Cautions

1.For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.

2.Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.

3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.

4.For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

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